Antigenic Proteins Involved in Occupational Rhinitis 1 and Asthma Caused by Obeche Wood (Triplochiton Scleroxylon)

Aranda, Ana and Campo, Paloma and Palacín Gómez, Aranzazu and Doña, Inmaculada and Gomez Casado, Cristina and Galindo, Luisa and Díaz Perales, Araceli and Blanca, Miguel (2013). Antigenic Proteins Involved in Occupational Rhinitis 1 and Asthma Caused by Obeche Wood (Triplochiton Scleroxylon). "Plos One", v. 8 (n. 1); pp.. ISSN 1932-6203. https://doi.org/10.1371/journal.pone.0053926.

Description

Title: Antigenic Proteins Involved in Occupational Rhinitis 1 and Asthma Caused by Obeche Wood (Triplochiton Scleroxylon)
Author/s:
  • Aranda, Ana
  • Campo, Paloma
  • Palacín Gómez, Aranzazu
  • Doña, Inmaculada
  • Gomez Casado, Cristina
  • Galindo, Luisa
  • Díaz Perales, Araceli
  • Blanca, Miguel
Item Type: Article
Título de Revista/Publicación: Plos One
Date: January 2013
ISSN: 1932-6203
Volume: 8
Subjects:
Faculty: E.T.S.I. Agrónomos (UPM) [antigua denominación]
Department: Biotecnologia [hasta 2014]
Creative Commons Licenses: Recognition - No derivative works - Non commercial

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Abstract

Background Obeche wood dust is a known cause of occupational asthma where an IgE-mediated mechanism has been demonstrated. Objective To characterize the allergenic profile of obeche wood dust and evaluate the reactivity of the proteins by in vitro, ex vivo and in vivo assays in carpenters with confirmed rhinitis and/or asthma Materials and methods An in-house obeche extract was obtained, and two IgE binding bands were purified (24 and 12 kDa) and sequenced by N-terminal identity. Specific IgE and IgG, basophil activation tests and skin prick tests (SPTs) were performed with whole extract and purified proteins. CCD binding was analyzed by ELISA inhibition studies. Results Sixty-two subjects participated: 12 with confirmed occupational asthma/rhinitis (ORA+), 40 asymptomatic exposed (ORA−), and 10 controls. Of the confirmed subjects, 83% had a positive SPT to obeche. There was a 100% recognition by ELISA in symptomatic subjects vs. 30% and 10% in asymptomatic exposed subjects and controls respectively (p<0.05). Two new proteins were purified, a 24 kDa protein identified as a putative thaumatin-like protein and a 12 kDa gamma-expansin. Both showed allergenic activity in vitro, with the putative thaumatin being the most active, with 92% recognition by ELISA and 100% by basophil activation test in ORA+ subjects. Cross-reactivity due to CCD was ruled out in 82% of cases. Conclusions Two proteins of obeche wood were identified and were recognized by a high percentage of symptomatic subjects and by a small proportion of asymptomatic exposed subjects. Further studies are required to evaluate cross reactivity with other plant allergens.

More information

Item ID: 19098
DC Identifier: http://oa.upm.es/19098/
OAI Identifier: oai:oa.upm.es:19098
DOI: 10.1371/journal.pone.0053926
Official URL: http://www.plosone.org/article/info:doi/10.1371/journal.pone.0053926
Deposited by: Memoria Investigacion
Deposited on: 06 Sep 2013 13:11
Last Modified: 21 Apr 2016 17:20
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