The AtCathB3 gene, encoding a cathepsin B-like protease, is expressed during germination of Arabidopsis thaliana and transcriptionally repressed by the basic leucine zipper protein GBF1.

Iglesias Fernandez, Raquel and Wozny, Dorothee and Iriondo de Hond, Maite and Oñate Sanchez, Luis and Carbonero Zalduegui, Pilar and Barrero Sicilia, Cristina (2013). The AtCathB3 gene, encoding a cathepsin B-like protease, is expressed during germination of Arabidopsis thaliana and transcriptionally repressed by the basic leucine zipper protein GBF1.. "Journal of Experimental Botany", v. 65 (n. 8); pp. 2009-2021. ISSN 0022-0957. https://doi.org/10.1093/jxb/eru055.

Description

Title: The AtCathB3 gene, encoding a cathepsin B-like protease, is expressed during germination of Arabidopsis thaliana and transcriptionally repressed by the basic leucine zipper protein GBF1.
Author/s:
  • Iglesias Fernandez, Raquel
  • Wozny, Dorothee
  • Iriondo de Hond, Maite
  • Oñate Sanchez, Luis
  • Carbonero Zalduegui, Pilar
  • Barrero Sicilia, Cristina
Item Type: Article
Título de Revista/Publicación: Journal of Experimental Botany
Date: March 2013
ISSN: 0022-0957
Volume: 65
Subjects:
Faculty: Centro de Investigación en Biotecnología y Genómica de Plantas (CBGP) (UPM)
Department: Biotecnologia [hasta 2014]
Creative Commons Licenses: Recognition - No derivative works - Non commercial

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Abstract

Protein hydrolysis plays an important role during seed germination and post-germination seedling establishment. In Arabidopsis thaliana, cathepsin B-like proteases are encoded by a gene family of three members, but only the AtCathB3 gene is highly induced upon seed germination and at the early post-germination stage. Seeds of a homozygous T-DNA insertion mutant in the AtCathB3 gene have, besides a reduced cathepsin B activity, a slower germination than the wild type. To explore the transcriptional regulation of this gene, we used a combined phylogenetic shadowing approach together with a yeast one-hybrid screening of an arrayed library of approximately 1200 transcription factor open reading frames from Arabidopsis thaliana. We identified a conserved CathB3-element in the promoters of orthologous CathB3 genes within the Brassicaceae species analysed, and, as its DNA-interacting protein, the G-Box Binding Factor1 (GBF1). Transient overexpression of GBF1 together with a PAtCathB3::uidA (β-glucuronidase) construct in tobacco plants revealed a negative effect of GBF1 on expression driven by the AtCathB3 promoter. In stable P35S::GBF1 lines, not only was the expression of the AtCathB3 gene drastically reduced, but a significant slower germination was also observed. In the homozygous knockout mutant for the GBF1 gene, the opposite effect was found. These data indicate that GBF1 is a transcriptional repressor of the AtCathB3 gene and affects the germination kinetics of Arabidopsis thaliana seeds. As AtCathB3 is also expressed during post-germination in the cotyledons, a role for the AtCathB3-like protease in reserve mobilization is also inferred.

More information

Item ID: 26422
DC Identifier: http://oa.upm.es/26422/
OAI Identifier: oai:oa.upm.es:26422
DOI: 10.1093/jxb/eru055
Official URL: http://jxb.oxfordjournals.org/content/65/8/2009.abstract
Deposited by: Memoria Investigacion
Deposited on: 29 Jul 2014 13:38
Last Modified: 31 May 2015 22:56
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