The AtCathB3 gene, encoding a cathepsin B-like protease, is expressed during germination of Arabidopsis thaliana and transcriptionally repressed by the basic leucine zipper protein GBF1.

Iglesias Fernandez, Raquel; Wozny, Dorothee; Iriondo de Hond, Maite; Oñate Sanchez, Luis; Carbonero Zalduegui, Pilar y Barrero Sicilia, Cristina (2013). The AtCathB3 gene, encoding a cathepsin B-like protease, is expressed during germination of Arabidopsis thaliana and transcriptionally repressed by the basic leucine zipper protein GBF1.. "Journal of Experimental Botany", v. 65 (n. 8); pp. 2009-2021. ISSN 0022-0957. https://doi.org/10.1093/jxb/eru055.

Descripción

Título: The AtCathB3 gene, encoding a cathepsin B-like protease, is expressed during germination of Arabidopsis thaliana and transcriptionally repressed by the basic leucine zipper protein GBF1.
Autor/es:
  • Iglesias Fernandez, Raquel
  • Wozny, Dorothee
  • Iriondo de Hond, Maite
  • Oñate Sanchez, Luis
  • Carbonero Zalduegui, Pilar
  • Barrero Sicilia, Cristina
Tipo de Documento: Artículo
Título de Revista/Publicación: Journal of Experimental Botany
Fecha: Marzo 2013
Volumen: 65
Materias:
Escuela: Centro de Investigación en Biotecnología y Genómica de Plantas (CBGP) (UPM)
Departamento: Biotecnologia [hasta 2014]
Licencias Creative Commons: Reconocimiento - Sin obra derivada - No comercial

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Resumen

Protein hydrolysis plays an important role during seed germination and post-germination seedling establishment. In Arabidopsis thaliana, cathepsin B-like proteases are encoded by a gene family of three members, but only the AtCathB3 gene is highly induced upon seed germination and at the early post-germination stage. Seeds of a homozygous T-DNA insertion mutant in the AtCathB3 gene have, besides a reduced cathepsin B activity, a slower germination than the wild type. To explore the transcriptional regulation of this gene, we used a combined phylogenetic shadowing approach together with a yeast one-hybrid screening of an arrayed library of approximately 1200 transcription factor open reading frames from Arabidopsis thaliana. We identified a conserved CathB3-element in the promoters of orthologous CathB3 genes within the Brassicaceae species analysed, and, as its DNA-interacting protein, the G-Box Binding Factor1 (GBF1). Transient overexpression of GBF1 together with a PAtCathB3::uidA (β-glucuronidase) construct in tobacco plants revealed a negative effect of GBF1 on expression driven by the AtCathB3 promoter. In stable P35S::GBF1 lines, not only was the expression of the AtCathB3 gene drastically reduced, but a significant slower germination was also observed. In the homozygous knockout mutant for the GBF1 gene, the opposite effect was found. These data indicate that GBF1 is a transcriptional repressor of the AtCathB3 gene and affects the germination kinetics of Arabidopsis thaliana seeds. As AtCathB3 is also expressed during post-germination in the cotyledons, a role for the AtCathB3-like protease in reserve mobilization is also inferred.

Más información

ID de Registro: 26422
Identificador DC: http://oa.upm.es/26422/
Identificador OAI: oai:oa.upm.es:26422
Identificador DOI: 10.1093/jxb/eru055
URL Oficial: http://jxb.oxfordjournals.org/content/65/8/2009.abstract
Depositado por: Memoria Investigacion
Depositado el: 29 Jul 2014 13:38
Ultima Modificación: 31 May 2015 22:56
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