Cis-, trans-, transcriptional regulation of the Arabidopsis thaliana gene AtTrxo1 and its response upon germination and to salt

Ortiz Espín, Ana María and Iglesias Fernandez, Raquel and Martínez Alcalá, Isabel María and Calderón Pérez, Aingeru and Camejo López, Daymi and Sevilla, Francisca and Carbonero Zalduegui, Pilar and Jimenez, Ana (2013). Cis-, trans-, transcriptional regulation of the Arabidopsis thaliana gene AtTrxo1 and its response upon germination and to salt. In: "11th International POG Conference: Reactive oxygen and nitrogen species in plants", 17/07/2013 - 19/07/2013, Varsovia (Polonia). p. 558.

Description

Title: Cis-, trans-, transcriptional regulation of the Arabidopsis thaliana gene AtTrxo1 and its response upon germination and to salt
Author/s:
  • Ortiz Espín, Ana María
  • Iglesias Fernandez, Raquel
  • Martínez Alcalá, Isabel María
  • Calderón Pérez, Aingeru
  • Camejo López, Daymi
  • Sevilla, Francisca
  • Carbonero Zalduegui, Pilar
  • Jimenez, Ana
Item Type: Presentation at Congress or Conference (Poster)
Event Title: 11th International POG Conference: Reactive oxygen and nitrogen species in plants
Event Dates: 17/07/2013 - 19/07/2013
Event Location: Varsovia (Polonia)
Title of Book: Proceedings of 11th International POG Conference: Reactive oxygen and nitrogen species in plants
Date: July 2013
Subjects:
Faculty: Centro de Investigación en Biotecnología y Genómica de Plantas (CBGP) (UPM)
Department: Biotecnologia [hasta 2014]
Creative Commons Licenses: Recognition - No derivative works - Non commercial

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Abstract

Plants contain several genes encoding thioredoxins (Trxs), small proteins involved in redox regulation of many enzymes in different cell compartments. Among them, mitochondrial Trxo has been described to have a response in plants grown under salinity but there is scarce information about its functional role in abiotic stress or its gene regulation. In this work, the transcriptional regulation of the mitochondrial AtTrxo1 gene has been studied for the first time, by identifying functionally relevant cis- elements in its promoter: two conserved motives were found as positive and one as negative regulators. Using them as baits for the screening of an arrayed yeast library containing Arabidopsis Transcription Factors (TF) ORFs, two TFs were selected that are now being validated at the molecular level. We have also studied the response of T-DNA insertion mutant plants for AtTrxo1 to salt stress. The K.O. AtTrxo1 mutants presented several phenotypic changes including the time required to reach 50% germination under salinity, without affecting the final germination percentage.

More information

Item ID: 26465
DC Identifier: http://oa.upm.es/26465/
OAI Identifier: oai:oa.upm.es:26465
Deposited by: Memoria Investigacion
Deposited on: 09 May 2015 07:50
Last Modified: 09 May 2015 07:50
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