Cis-, trans-, transcriptional regulation of the Arabidopsis thaliana gene AtTrxo1 and its response upon germination and to salt

Ortiz Espín, Ana María; Iglesias Fernandez, Raquel; Martínez Alcalá, Isabel María; Calderón Pérez, Aingeru; Camejo López, Daymi; Sevilla, Francisca; Carbonero Zalduegui, Pilar y Jimenez, Ana (2013). Cis-, trans-, transcriptional regulation of the Arabidopsis thaliana gene AtTrxo1 and its response upon germination and to salt. En: "11th International POG Conference: Reactive oxygen and nitrogen species in plants", 17/07/2013 - 19/07/2013, Varsovia (Polonia). p. 558.

Descripción

Título: Cis-, trans-, transcriptional regulation of the Arabidopsis thaliana gene AtTrxo1 and its response upon germination and to salt
Autor/es:
  • Ortiz Espín, Ana María
  • Iglesias Fernandez, Raquel
  • Martínez Alcalá, Isabel María
  • Calderón Pérez, Aingeru
  • Camejo López, Daymi
  • Sevilla, Francisca
  • Carbonero Zalduegui, Pilar
  • Jimenez, Ana
Tipo de Documento: Ponencia en Congreso o Jornada (Póster)
Título del Evento: 11th International POG Conference: Reactive oxygen and nitrogen species in plants
Fechas del Evento: 17/07/2013 - 19/07/2013
Lugar del Evento: Varsovia (Polonia)
Título del Libro: Proceedings of 11th International POG Conference: Reactive oxygen and nitrogen species in plants
Fecha: Julio 2013
Materias:
Escuela: Centro de Investigación en Biotecnología y Genómica de Plantas (CBGP) (UPM)
Departamento: Biotecnologia [hasta 2014]
Licencias Creative Commons: Reconocimiento - Sin obra derivada - No comercial

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Resumen

Plants contain several genes encoding thioredoxins (Trxs), small proteins involved in redox regulation of many enzymes in different cell compartments. Among them, mitochondrial Trxo has been described to have a response in plants grown under salinity but there is scarce information about its functional role in abiotic stress or its gene regulation. In this work, the transcriptional regulation of the mitochondrial AtTrxo1 gene has been studied for the first time, by identifying functionally relevant cis- elements in its promoter: two conserved motives were found as positive and one as negative regulators. Using them as baits for the screening of an arrayed yeast library containing Arabidopsis Transcription Factors (TF) ORFs, two TFs were selected that are now being validated at the molecular level. We have also studied the response of T-DNA insertion mutant plants for AtTrxo1 to salt stress. The K.O. AtTrxo1 mutants presented several phenotypic changes including the time required to reach 50% germination under salinity, without affecting the final germination percentage.

Más información

ID de Registro: 26465
Identificador DC: http://oa.upm.es/26465/
Identificador OAI: oai:oa.upm.es:26465
Depositado por: Memoria Investigacion
Depositado el: 09 May 2015 07:50
Ultima Modificación: 09 May 2015 07:50
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