Transcription factors of the bzip factors of the BZIP family affecting arabidopsis thaliana seed germination sensu stricto

Iglesias Fernandez, Raquel and Barrero Sicilia, Cristina and Carrillo Barral, Nestor and Oñate Sanchez, Luis and Carbonero Zalduegui, Pilar (2013). Transcription factors of the bzip factors of the BZIP family affecting arabidopsis thaliana seed germination sensu stricto. In: "XIII Congresso Luso - Espanhol de Fisiologia Vegetal", 24/07/2013 - 28/07/2013, Lisbon, Portugal. p. 115.

Description

Title: Transcription factors of the bzip factors of the BZIP family affecting arabidopsis thaliana seed germination sensu stricto
Author/s:
  • Iglesias Fernandez, Raquel
  • Barrero Sicilia, Cristina
  • Carrillo Barral, Nestor
  • Oñate Sanchez, Luis
  • Carbonero Zalduegui, Pilar
Item Type: Presentation at Congress or Conference (Poster)
Event Title: XIII Congresso Luso - Espanhol de Fisiologia Vegetal
Event Dates: 24/07/2013 - 28/07/2013
Event Location: Lisbon, Portugal
Title of Book: Transcription factors of the bzip factors of the BZIP family affecting arabidopsis thaliana germination sensu stricto
Date: 2013
Subjects:
Faculty: Centro de Investigación en Biotecnología y Genómica de Plantas (CBGP) (UPM)
Department: Biotecnologia [hasta 2014]
Creative Commons Licenses: Recognition - No derivative works - Non commercial

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Abstract

During seed germination, the endosperm cell walls (CWs) suffer an important weakening process mainly driven by hydrolytic enzymes, such are endo-?- mannanases (MAN; EC. 3.2.1.78) that catalyze the cleavage of ?1?4 bonds in the mannan-polymers. In Arabidopsis thaliana seeds, endo-?-mannanase activity increases during seed imbibition, decreasing after radicle emergence1. AtMAN7 is the most highly expressed MAN gene in seeds upon germination and their transcripts are restricted to the micropylar endosperm and to the radicle tip just before radicle emergence. Mutants with a T-DNA insertion in this gene (K.O. MAN7) have a slower germination rate than the wild type (t50=34 h versus t50=25 h). To gain insight into the transcriptional regulation of the AtMAN7 gene, a bioinformatic search for conserved non-coding cis-elements (phylogenetic shadowing) within the Brassicaceae orthologous MAN7 gene promoters has been done and these conserved motives have been used as baits to look for their interacting transcription factors (TFs), using as a prey an arrayed yeast library of circa 1,200 TFs from A. thaliana. The basic leucine zipper AtbZIP44, but not its closely related ortholog AtbZIP11, has been thus identified and its regulatory function upon AtMAN7 during seed germination validated by different molecular and physiological techniques, such are RT-qPCR analyses, mRNA Fluorescence in situ Hybridization (FISH) experiments, and by the establishment of the germination kinetics of both over-expression (oex) lines and TDNA insertion mutants in AtbZIP44. The transcriptional combinatorial network through which AtbZIP44 regulates AtMAN7 gene expression during seed germination has been further explored through protein-protein interactions between AtbZIP44 and other bZIP members. In such a way, AtbZIP9 has been identified by yeast two-hybrid experiments and its physiological implication in the control of AtMAN7 expression similarly established.

More information

Item ID: 26470
DC Identifier: http://oa.upm.es/26470/
OAI Identifier: oai:oa.upm.es:26470
Deposited by: Memoria Investigacion
Deposited on: 05 May 2015 18:22
Last Modified: 29 Oct 2018 14:59
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