Xyloglucan endotransglucosylase/hydrolase (XTH) overexpression affects growth and cell wall mechanics in etiolated Arabidopsis hypocotyls.

Miedes Vicente, Eva and Suslov, Dmitry and Vandenbussche, Filip and Kenobi, Kim and Ivakov, Alexander and Van Der Straeten, Dominique and Lorences, E. P. and Mellerowicz, Ewa J. and Verbelen, Jean Pierre and Vissenberg, Kris (2013). Xyloglucan endotransglucosylase/hydrolase (XTH) overexpression affects growth and cell wall mechanics in etiolated Arabidopsis hypocotyls.. "Journal of Experimental Botany", v. 64 (n. 8); pp. 2481-2497. ISSN 0022-0957. https://doi.org/10.1093/jxb/ert107.

Description

Title: Xyloglucan endotransglucosylase/hydrolase (XTH) overexpression affects growth and cell wall mechanics in etiolated Arabidopsis hypocotyls.
Author/s:
  • Miedes Vicente, Eva
  • Suslov, Dmitry
  • Vandenbussche, Filip
  • Kenobi, Kim
  • Ivakov, Alexander
  • Van Der Straeten, Dominique
  • Lorences, E. P.
  • Mellerowicz, Ewa J.
  • Verbelen, Jean Pierre
  • Vissenberg, Kris
Item Type: Article
Título de Revista/Publicación: Journal of Experimental Botany
Date: 2013
ISSN: 0022-0957
Volume: 64
Subjects:
Faculty: Centro de Investigación en Biotecnología y Genómica de Plantas (CBGP) (UPM)
Department: Biotecnologia [hasta 2014]
Creative Commons Licenses: Recognition - No derivative works - Non commercial

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Abstract

Growth and biomechanics of etiolated hypocotyls from Arabidopsis thaliana lines overexpressing xyloglucan endotransglucosylase/hydrolase AtXTH18, AtXTH19, AtXTH20, and PttXET16-34 were studied. Overexpression of AtXTH18, AtXTH19, and AtXTH20 stimulated growth of hypocotyls, while PttXET16-34 overexpression did not show this effect. In vitro extension of frozen/thawed hypocotyls measured by a constant-load extensiometer started from a high-amplitude initial deformation followed by a slow time-dependent creep. Creep of growing XTH-overexpressing (OE) hypocotyls was more linear in time compared with the wild type at pH 5.0, reflecting their higher potential for long-term extension. XTH-OE plants deposited 65?84% more cell wall material per hypocotyl cross-sectional area than wild-type plants. As a result, their wall stress under each external load was lower than in the wild-type. Growing XTH-OE hypocotyls had higher values of initial deformation·stress?1 compared with the wild type. Plotting creep rates for each line under different loads against the respective wall stress values gave straight lines. Their slopes and intercepts with the abscissa correspond to ? (in vitro cell wall extensibility) and y (in vitro cell wall yield threshold) values characterizing cell wall material properties. The wall material in XTH-OE lines was more pliant than in the wild type due to lower y values. In contrast, the acid-induced wall extension in vitro resulted from increasing ? values. Thus, three factors contributed to the XTH-OE-stimulated growth in Arabidopsis hypocotyls: their more linear creep, higher values of initial deformation·stress?1, and lower y values.

More information

Item ID: 29012
DC Identifier: http://oa.upm.es/29012/
OAI Identifier: oai:oa.upm.es:29012
DOI: 10.1093/jxb/ert107
Official URL: http://jxb.oxfordjournals.org/content/64/8/2481.abstract
Deposited by: Memoria Investigacion
Deposited on: 29 Jul 2014 15:02
Last Modified: 22 Sep 2014 11:43
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