Chemical and structural analysis of Eucalyptus globulus and E. camaldulensis leaf cuticles: a lipidized cell wall region

Guzmán Delgado, Paula; Fernandez Fernandez, Victoria; Graça, José; Cabral, Vanessa; Khayet, Mohamed y Gil Sanchez, Luis (2014). Chemical and structural analysis of Eucalyptus globulus and E. camaldulensis leaf cuticles: a lipidized cell wall region. "Frontiers in Plant Science", v. 5 (n. 481); pp. 1-12. https://doi.org/10.3389/fpls.2014.00481.

Descripción

Título: Chemical and structural analysis of Eucalyptus globulus and E. camaldulensis leaf cuticles: a lipidized cell wall region
Autor/es:
  • Guzmán Delgado, Paula
  • Fernandez Fernandez, Victoria
  • Graça, José
  • Cabral, Vanessa
  • Khayet, Mohamed
  • Gil Sanchez, Luis
Tipo de Documento: Artículo
Título de Revista/Publicación: Frontiers in Plant Science
Fecha: 16 Septiembre 2014
Volumen: 5
Materias:
Palabras Clave Informales: Electron microscopy; Cuticle; Cutin; Infrared spectroscopy; Gas chromatography-Mass spectrometry; Leaf; Polysaccharides; Waxes
Escuela: E.T.S.I. Montes, Forestal y del Medio Natural (UPM)
Departamento: Silvopascicultura [hasta 2014]
Licencias Creative Commons: Reconocimiento - No comercial

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Resumen

The plant cuticle has traditionally been conceived as an independent hydrophobic layer that covers the external epidermal cell wall. Due to its complexity, the existing relationship between cuticle chemical composition and ultra-structure remains unclear to date. This study aimed to examine the link between chemical composition and structure of isolated, adaxial leaf cuticles of Eucalyptus camaldulensis and E. globulus by the gradual extraction and identification of lipid constituents (cutin and soluble lipids), coupled to spectroscopic and microscopic analyses. The soluble compounds and cutin monomers identified could not be assigned to a concrete internal cuticle ultra-structure. After cutin depolymerization, a cellulose network resembling the cell wall was observed, with different structural patterns in the regions ascribed to the cuticle proper and cuticular layer, respectively. Our results suggest that the current cuticle model should be revised, stressing the presence and major role of cell wall polysaccharides. It is concluded that the cuticle may be interpreted as a modified cell wall region which contains additional lipids. The major heterogeneity of the plant cuticle makes it difficult to establish a direct link between cuticle chemistry and structure with the existing methodologies.

Más información

ID de Registro: 30952
Identificador DC: http://oa.upm.es/30952/
Identificador OAI: oai:oa.upm.es:30952
Identificador DOI: 10.3389/fpls.2014.00481
URL Oficial: http://journal.frontiersin.org/Journal/10.3389/fpls.2014.00481/abstract
Depositado por: Victoria Fernández
Depositado el: 17 Sep 2014 06:50
Ultima Modificación: 26 Sep 2014 08:48
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