Chemical and structural analysis of Eucalyptus globulus and E. camaldulensis leaf cuticles: a lipidized cell wall region

Guzmán Delgado, Paula and Fernandez Fernandez, Victoria and Graça, José and Cabral, Vanessa and Khayet, Mohamed and Gil Sanchez, Luis Alfonso (2014). Chemical and structural analysis of Eucalyptus globulus and E. camaldulensis leaf cuticles: a lipidized cell wall region. "Frontiers in Plant Science", v. 5 (n. 481); pp. 1-12. https://doi.org/10.3389/fpls.2014.00481.

Description

Title: Chemical and structural analysis of Eucalyptus globulus and E. camaldulensis leaf cuticles: a lipidized cell wall region
Author/s:
  • Guzmán Delgado, Paula
  • Fernandez Fernandez, Victoria
  • Graça, José
  • Cabral, Vanessa
  • Khayet, Mohamed
  • Gil Sanchez, Luis Alfonso
Item Type: Article
Título de Revista/Publicación: Frontiers in Plant Science
Date: 16 September 2014
Volume: 5
Subjects:
Freetext Keywords: Electron microscopy; Cuticle; Cutin; Infrared spectroscopy; Gas chromatography-Mass spectrometry; Leaf; Polysaccharides; Waxes
Faculty: E.T.S.I. Montes, Forestal y del Medio Natural (UPM)
Department: Silvopascicultura [hasta 2014]
Creative Commons Licenses: Recognition - Non commercial

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Abstract

The plant cuticle has traditionally been conceived as an independent hydrophobic layer that covers the external epidermal cell wall. Due to its complexity, the existing relationship between cuticle chemical composition and ultra-structure remains unclear to date. This study aimed to examine the link between chemical composition and structure of isolated, adaxial leaf cuticles of Eucalyptus camaldulensis and E. globulus by the gradual extraction and identification of lipid constituents (cutin and soluble lipids), coupled to spectroscopic and microscopic analyses. The soluble compounds and cutin monomers identified could not be assigned to a concrete internal cuticle ultra-structure. After cutin depolymerization, a cellulose network resembling the cell wall was observed, with different structural patterns in the regions ascribed to the cuticle proper and cuticular layer, respectively. Our results suggest that the current cuticle model should be revised, stressing the presence and major role of cell wall polysaccharides. It is concluded that the cuticle may be interpreted as a modified cell wall region which contains additional lipids. The major heterogeneity of the plant cuticle makes it difficult to establish a direct link between cuticle chemistry and structure with the existing methodologies.

More information

Item ID: 30952
DC Identifier: http://oa.upm.es/30952/
OAI Identifier: oai:oa.upm.es:30952
DOI: 10.3389/fpls.2014.00481
Official URL: http://journal.frontiersin.org/Journal/10.3389/fpls.2014.00481/abstract
Deposited by: Victoria Fernández
Deposited on: 17 Sep 2014 06:50
Last Modified: 10 Jan 2019 13:02
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