A non-persistently transmitted-virus induces a pull?push strategy inits aphid vector to optimize transmission and spread

Sousa Timossi, Michele Do Carmo and Moreno, Aranzazu and Garz, Elisa and Fereres Castiel, Alberto (2014). A non-persistently transmitted-virus induces a pull?push strategy inits aphid vector to optimize transmission and spread. "Virus Research", v. 186 ; pp. 38-46. ISSN 0168-1702. https://doi.org/10.1016/S0168-1702(14)00187-7.

Description

Title: A non-persistently transmitted-virus induces a pull?push strategy inits aphid vector to optimize transmission and spread
Author/s:
  • Sousa Timossi, Michele Do Carmo
  • Moreno, Aranzazu
  • Garz, Elisa
  • Fereres Castiel, Alberto
Item Type: Article
Título de Revista/Publicación: Virus Research
Date: June 2014
ISSN: 0168-1702
Volume: 186
Subjects:
Faculty: E.T.S.I. Agrónomos (UPM) [antigua denominación]
Department: Otro
Creative Commons Licenses: Recognition - No derivative works - Non commercial

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Abstract

Plant viruses are known to modify the behaviour of their insect vectors, both directly and indirectly,generally adapting to each type of virus?vector relationship in a way that enhances transmissionefficiency. Here, we report results of three different studies showing how a virus transmitted in a non-persistent (NP) manner (Cucumber mosaic virus; CMV, Cucumovirus) can induce changes in its host plant,cucumber (Cucumis sativus cv. Marumba) that modifies the behaviour of its aphid vector (Aphis gossypiiGlover; Hemiptera: Aphididae) in a way that enhances virus transmission and spread non-viruliferousaphids changed their alighting, settling and probing behaviour activities over time when exposed toCMV-infected and mock-inoculated cucumber plants. Aphids exhibited no preference to migrate fromCMV-infected to mock-inoculated plants at short time intervals (1, 10 and 30 min after release), butshowed a clear shift in preference to migrate from CMV-infected to mock-inoculated plants 60 min afterrelease. Our free-choice preference assays showed that A. gossypii alates preferred CMV-infected overmock-inoculated plants at an early stage (30 min), but this behaviour was reverted at a later stage andaphids preferred to settle and reproduce on mock-inoculated plants. The electrical penetration graph(EPG) technique revealed a sharp change in aphid probing behaviour over time when exposed to CMV-infected plants. At the beginning (first 15 min) aphid vectors dramatically increased the number of shortsuperficial probes and intracellular punctures when exposed to CMV-infected plants. At a later stage (sec-ond hour of recording) aphids diminished their feeding on CMV-infected plants as indicated by much lesstime spent in phloem salivation and ingestion (E1 and E2). This particular probing behaviour includingan early increase in the number of short superficial probes and intracellular punctures followed by aphloem feeding deterrence is known to enhance the transmission efficiency of viruses transmitted in aNP manner. We conclude that CMV induces specific changes in a plant host that modify the alighting,settling and probing behaviour of its main vector A. gossypii, leading to optimum transmission and spreadof the virus. Our findings should be considered when modelling the spread of viruses transmitted in a NPmanner.

Funding Projects

TypeCodeAcronymLeaderTitle
Government of SpainAGL2010-22196-C02-01UnspecifiedUnspecifiedUnspecified

More information

Item ID: 35007
DC Identifier: http://oa.upm.es/35007/
OAI Identifier: oai:oa.upm.es:35007
DOI: 10.1016/S0168-1702(14)00187-7
Official URL: http://www.sciencedirect.com/science/article/pii/S0168170213004413
Deposited by: Memoria Investigacion
Deposited on: 04 May 2015 15:28
Last Modified: 06 May 2019 10:40
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