Three-dimensional distribution of cortical synapses: a replicated point pattern-based analysis

Antón Sánchez, Laura; Bielza Lozoya, Maria Concepcion; Merchán Perez, Ángel; Rodríguez Sánchez, José Rodrigo; De Felipe Oroquieta, Javier y Larrañaga Múgica, Pedro (2014). Three-dimensional distribution of cortical synapses: a replicated point pattern-based analysis. "Frontiers in Neuroanatomy", v. 8 (n. null); pp. 1-15. ISSN 1662-5129. https://doi.org/10.3389/fnana.2014.00085.

Descripción

Título: Three-dimensional distribution of cortical synapses: a replicated point pattern-based analysis
Autor/es:
  • Antón Sánchez, Laura
  • Bielza Lozoya, Maria Concepcion
  • Merchán Perez, Ángel
  • Rodríguez Sánchez, José Rodrigo
  • De Felipe Oroquieta, Javier
  • Larrañaga Múgica, Pedro
Tipo de Documento: Artículo
Título de Revista/Publicación: Frontiers in Neuroanatomy
Fecha: Agosto 2014
Volumen: 8
Materias:
Palabras Clave Informales: Spatial distribution of synapses; Neocortex; Dual-beam electron microscopy; FIB/SEM; Replicated spatial point patterns; random sequential adsorption; 3D Ripley's K function; Besag's L function
Escuela: E.T.S. de Ingenieros Informáticos (UPM)
Departamento: Inteligencia Artificial
Licencias Creative Commons: Reconocimiento - Sin obra derivada - No comercial

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Resumen

The biggest problem when analyzing the brain is that its synaptic connections are extremely complex. Generally, the billions of neurons making up the brain exchange information through two types of highly specialized structures: chemical synapses (the vast majority) and so-called gap junctions (a substrate of one class of electrical synapse). Here we are interested in exploring the three-dimensional spatial distribution of chemical synapses in the cerebral cortex. Recent research has showed that the three-dimensional spatial distribution of synapses in layer III of the neocortex can be modeled by a random sequential adsorption (RSA) point process, i.e., synapses are distributed in space almost randomly, with the only constraint that they cannot overlap. In this study we hypothesize that RSA processes can also explain the distribution of synapses in all cortical layers. We also investigate whether there are differences in both the synaptic density and spatial distribution of synapses between layers. Using combined focused ion beam milling and scanning electron microscopy (FIB/SEM), we obtained three-dimensional samples from the six layers of the rat somatosensory cortex and identified and reconstructed the synaptic junctions. A total volume of tissue of approximately 4500μm3 and around 4000 synapses from three different animals were analyzed. Different samples, layers and/or animals were aggregated and compared using RSA replicated spatial point processes. The results showed no significant differences in the synaptic distribution across the different rats used in the study. We found that RSA processes described the spatial distribution of synapses in all samples of each layer. We also found that the synaptic distribution in layers II to VI conforms to a common underlying RSA process with different densities per layer. Interestingly, the results showed that synapses in layer I had a slightly different spatial distribution from the other layers.

Más información

ID de Registro: 35439
Identificador DC: http://oa.upm.es/35439/
Identificador OAI: oai:oa.upm.es:35439
Identificador DOI: 10.3389/fnana.2014.00085
URL Oficial: http://journal.frontiersin.org/article/10.3389/fnana.2014.00085/abstract
Depositado por: Memoria Investigacion
Depositado el: 09 Jun 2015 12:27
Ultima Modificación: 30 Oct 2017 11:39
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