Three-dimensional distribution of cortical synapses: a replicated point pattern-based analysis

Antón Sánchez, Laura and Bielza Lozoya, Maria Concepcion and Merchán Perez, Ángel and Rodríguez Sánchez, José Rodrigo and De Felipe Oroquieta, Javier and Larrañaga Múgica, Pedro (2014). Three-dimensional distribution of cortical synapses: a replicated point pattern-based analysis. "Frontiers in Neuroanatomy", v. 8 (n. null); pp. 1-15. ISSN 1662-5129. https://doi.org/10.3389/fnana.2014.00085.

Description

Title: Three-dimensional distribution of cortical synapses: a replicated point pattern-based analysis
Author/s:
  • Antón Sánchez, Laura
  • Bielza Lozoya, Maria Concepcion
  • Merchán Perez, Ángel
  • Rodríguez Sánchez, José Rodrigo
  • De Felipe Oroquieta, Javier
  • Larrañaga Múgica, Pedro
Item Type: Article
Título de Revista/Publicación: Frontiers in Neuroanatomy
Date: August 2014
Volume: 8
Subjects:
Freetext Keywords: Spatial distribution of synapses; Neocortex; Dual-beam electron microscopy; FIB/SEM; Replicated spatial point patterns; random sequential adsorption; 3D Ripley's K function; Besag's L function
Faculty: E.T.S. de Ingenieros Informáticos (UPM)
Department: Inteligencia Artificial
Creative Commons Licenses: Recognition - No derivative works - Non commercial

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Abstract

The biggest problem when analyzing the brain is that its synaptic connections are extremely complex. Generally, the billions of neurons making up the brain exchange information through two types of highly specialized structures: chemical synapses (the vast majority) and so-called gap junctions (a substrate of one class of electrical synapse). Here we are interested in exploring the three-dimensional spatial distribution of chemical synapses in the cerebral cortex. Recent research has showed that the three-dimensional spatial distribution of synapses in layer III of the neocortex can be modeled by a random sequential adsorption (RSA) point process, i.e., synapses are distributed in space almost randomly, with the only constraint that they cannot overlap. In this study we hypothesize that RSA processes can also explain the distribution of synapses in all cortical layers. We also investigate whether there are differences in both the synaptic density and spatial distribution of synapses between layers. Using combined focused ion beam milling and scanning electron microscopy (FIB/SEM), we obtained three-dimensional samples from the six layers of the rat somatosensory cortex and identified and reconstructed the synaptic junctions. A total volume of tissue of approximately 4500μm3 and around 4000 synapses from three different animals were analyzed. Different samples, layers and/or animals were aggregated and compared using RSA replicated spatial point processes. The results showed no significant differences in the synaptic distribution across the different rats used in the study. We found that RSA processes described the spatial distribution of synapses in all samples of each layer. We also found that the synaptic distribution in layers II to VI conforms to a common underlying RSA process with different densities per layer. Interestingly, the results showed that synapses in layer I had a slightly different spatial distribution from the other layers.

Funding Projects

TypeCodeAcronymLeaderTitle
Government of SpainTIN2013-41592-PUnspecifiedUnspecifiedUnspecified
Government of SpainBFU2012-34963UnspecifiedUnspecifiedUnspecified
Government of SpainSAF 2010-18218UnspecifiedUnspecifiedUnspecified
FP7604102HBPEcole Polytechnique Federale de LausanneThe Human Brain Project

More information

Item ID: 35439
DC Identifier: http://oa.upm.es/35439/
OAI Identifier: oai:oa.upm.es:35439
DOI: 10.3389/fnana.2014.00085
Official URL: http://journal.frontiersin.org/article/10.3389/fnana.2014.00085/abstract
Deposited by: Memoria Investigacion
Deposited on: 09 Jun 2015 12:27
Last Modified: 14 May 2019 11:13
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