Jasmonate-dependent modifications of the pectin matrix during potato development function as a defense mechanism targeted by Dickeya dadantii virulence factors

Taurino, Marco and Abelenda, José A. and Río-Álvarez, Isabel and Navarro, Cristina and Vicendo, Bogonya and Farmaki, Theodora and Jiménez, Pedro and García Agustín, Pilar and Lopez Solanilla, Emilia and Prat, Salomé and Rojo, Enrique and Sánchez Serrano, José J. and Sanmarti, M. (2014). Jasmonate-dependent modifications of the pectin matrix during potato development function as a defense mechanism targeted by Dickeya dadantii virulence factors. "Plant Journal", v. 77 (n. 3); pp. 418-429. ISSN 0960-7412. https://doi.org/10.1111/tpj.12393.

Description

Title: Jasmonate-dependent modifications of the pectin matrix during potato development function as a defense mechanism targeted by Dickeya dadantii virulence factors
Author/s:
  • Taurino, Marco
  • Abelenda, José A.
  • Río-Álvarez, Isabel
  • Navarro, Cristina
  • Vicendo, Bogonya
  • Farmaki, Theodora
  • Jiménez, Pedro
  • García Agustín, Pilar
  • Lopez Solanilla, Emilia
  • Prat, Salomé
  • Rojo, Enrique
  • Sánchez Serrano, José J.
  • Sanmarti, M.
Item Type: Article
Título de Revista/Publicación: Plant Journal
Date: 2014
ISSN: 0960-7412
Volume: 77
Subjects:
Faculty: Centro de Investigación en Biotecnología y Genómica de Plantas (CBGP) (UPM)
Department: Biotecnología - Biología Vegetal
Creative Commons Licenses: Recognition - No derivative works - Non commercial

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Abstract

The plant cell wall constitutes an essential protection barrier against pathogen attack. In addition, cell-wall disruption leads to accumulation of jasmonates (JAs), which are key signaling molecules for activation of plant inducible defense responses. However, whether JAs in return modulate the cell-wall composition to reinforce this defensive barrier remains unknown. The enzyme 13-allene oxide synthase (13-AOS) catalyzes the first committed step towards biosynthesis of JAs. In potato (Solanum tuberosum), there are two putative St13-AOS genes, which we show here to be differentially induced upon wounding. We also determine that both genes complement an Arabidopsis aos null mutant, indicating that they encode functional 13-AOS enzymes. Indeed, transgenic potato plants lacking both St13-AOS genes (CoAOS1/2 lines) exhibited a significant reduction of JAs, a concomitant decrease in wound-responsive gene activation, and an increased severity of soft rot disease symptoms caused by Dickeya dadantii. Intriguingly, a hypovirulent D. dadantii pel strain lacking the five major pectate lyases, which causes limited tissue maceration on wild-type plants, regained infectivity in CoAOS1/2 plants. In line with this, we found differences in pectin methyl esterase activity and cell-wall pectin composition between wild-type and CoAOS1/2 plants. Importantly, wild-type plants had pectins with a lower degree of methyl esterification, which are the substrates of the pectate lyases mutated in the pel strain. These results suggest that, during development of potato plants, JAs mediate modification of the pectin matrix to form a defensive barrier that is counteracted by pectinolytic virulence factors from D. dadantii.

Funding Projects

TypeCodeAcronymLeaderTitle
Government of SpainBIO2008‐03052UnspecifiedUnspecifiedUnspecified
Government of SpainBIO2009‐10784UnspecifiedUnspecifiedUnspecified
Government of SpainAGL‐2009‐12757UnspecifiedUnspecifiedUnspecified
Government of SpainBIO2008‐04160UnspecifiedUnspecifiedUnspecified
Government of SpainAGL2010‐22300UnspecifiedUnspecifiedUnspecified

More information

Item ID: 35599
DC Identifier: http://oa.upm.es/35599/
OAI Identifier: oai:oa.upm.es:35599
DOI: 10.1111/tpj.12393
Official URL: http://onlinelibrary.wiley.com/doi/10.1111/tpj.12393/full
Deposited by: Memoria Investigacion
Deposited on: 24 May 2016 15:31
Last Modified: 21 May 2019 09:51
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