Three-dimensional spatial distribution of synapses in the neocortex: A dual-beam electron microscopy study

Merchán Pérez, Ángel; Rodríguez Sánchez, José Rodrigo; González Tortosa, Santiago; Robles Forcada, Víctor; Felipe Oroquieta, Javier de; Larrañaga Múgica, Pedro y Bielza Lozoya, Maria Concepcion (2014). Three-dimensional spatial distribution of synapses in the neocortex: A dual-beam electron microscopy study. "Cerebral Cortex", v. 24 (n. 6); pp. 1579-1588. ISSN 1047-3211. https://doi.org/10.1093/cercor/bht018.

Descripción

Título: Three-dimensional spatial distribution of synapses in the neocortex: A dual-beam electron microscopy study
Autor/es:
  • Merchán Pérez, Ángel
  • Rodríguez Sánchez, José Rodrigo
  • González Tortosa, Santiago
  • Robles Forcada, Víctor
  • Felipe Oroquieta, Javier de
  • Larrañaga Múgica, Pedro
  • Bielza Lozoya, Maria Concepcion
Tipo de Documento: Artículo
Título de Revista/Publicación: Cerebral Cortex
Fecha: 2014
Volumen: 24
Materias:
Palabras Clave Informales: Dual-beam electron microscopy; Focused ion beam milling/ scanning electron microscopy; Neocortex; Spatial distribution of synapses; Spatial statistics; Synapses
Escuela: E.T.S. de Ingenieros Informáticos (UPM)
Departamento: Inteligencia Artificial
Licencias Creative Commons: Reconocimiento - Sin obra derivada - No comercial

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Resumen

In the cerebral cortex, most synapses are found in the neuropil, but relatively little is known about their 3-dimensional organization. Using an automated dual-beam electron microscope that combines focused ion beam milling and scanning electron microscopy, we have been able to obtain 10 three-dimensional samples with an average volume of 180 µm(3) from the neuropil of layer III of the young rat somatosensory cortex (hindlimb representation). We have used specific software tools to fully reconstruct 1695 synaptic junctions present in these samples and to accurately quantify the number of synapses per unit volume. These tools also allowed us to determine synapse position and to analyze their spatial distribution using spatial statistical methods. Our results indicate that the distribution of synaptic junctions in the neuropil is nearly random, only constrained by the fact that synapses cannot overlap in space. A theoretical model based on random sequential absorption, which closely reproduces the actual distribution of synapses, is also presented.

Más información

ID de Registro: 35609
Identificador DC: http://oa.upm.es/35609/
Identificador OAI: oai:oa.upm.es:35609
Identificador DOI: 10.1093/cercor/bht018
URL Oficial: http://cercor.oxfordjournals.org/content/by/year
Depositado por: Memoria Investigacion
Depositado el: 14 Jul 2015 09:34
Ultima Modificación: 14 Jul 2015 09:34
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