Three-dimensional spatial distribution of synapses in the neocortex: A dual-beam electron microscopy study

Merchán Pérez, Ángel and Rodríguez Sánchez, José Rodrigo and González Tortosa, Santiago and Robles Forcada, Víctor and Felipe Oroquieta, Javier de and Larrañaga Múgica, Pedro and Bielza Lozoya, Maria Concepcion (2014). Three-dimensional spatial distribution of synapses in the neocortex: A dual-beam electron microscopy study. "Cerebral Cortex", v. 24 (n. 6); pp. 1579-1588. ISSN 1047-3211. https://doi.org/10.1093/cercor/bht018.

Description

Title: Three-dimensional spatial distribution of synapses in the neocortex: A dual-beam electron microscopy study
Author/s:
  • Merchán Pérez, Ángel
  • Rodríguez Sánchez, José Rodrigo
  • González Tortosa, Santiago
  • Robles Forcada, Víctor
  • Felipe Oroquieta, Javier de
  • Larrañaga Múgica, Pedro
  • Bielza Lozoya, Maria Concepcion
Item Type: Article
Título de Revista/Publicación: Cerebral Cortex
Date: 2014
ISSN: 1047-3211
Volume: 24
Subjects:
Freetext Keywords: Dual-beam electron microscopy; Focused ion beam milling/ scanning electron microscopy; Neocortex; Spatial distribution of synapses; Spatial statistics; Synapses
Faculty: E.T.S. de Ingenieros Informáticos (UPM)
Department: Inteligencia Artificial
Creative Commons Licenses: Recognition - No derivative works - Non commercial

Full text

[img]
Preview
PDF - Requires a PDF viewer, such as GSview, Xpdf or Adobe Acrobat Reader
Download (4MB) | Preview

Abstract

In the cerebral cortex, most synapses are found in the neuropil, but relatively little is known about their 3-dimensional organization. Using an automated dual-beam electron microscope that combines focused ion beam milling and scanning electron microscopy, we have been able to obtain 10 three-dimensional samples with an average volume of 180 µm(3) from the neuropil of layer III of the young rat somatosensory cortex (hindlimb representation). We have used specific software tools to fully reconstruct 1695 synaptic junctions present in these samples and to accurately quantify the number of synapses per unit volume. These tools also allowed us to determine synapse position and to analyze their spatial distribution using spatial statistical methods. Our results indicate that the distribution of synaptic junctions in the neuropil is nearly random, only constrained by the fact that synapses cannot overlap in space. A theoretical model based on random sequential absorption, which closely reproduces the actual distribution of synapses, is also presented.

Funding Projects

TypeCodeAcronymLeaderTitle
Government of SpainSAF2009-09394UnspecifiedUnspecifiedUnspecified
Government of SpainSAF 2010-18218UnspecifiedUnspecifiedUnspecified
Government of SpainTIN2010-21289-C02-02UnspecifiedUnspecifiedUnspecified
Government of SpainTIN2010-20900-C04-04UnspecifiedUnspecifiedUnspecified

More information

Item ID: 35609
DC Identifier: http://oa.upm.es/35609/
OAI Identifier: oai:oa.upm.es:35609
DOI: 10.1093/cercor/bht018
Official URL: http://cercor.oxfordjournals.org/content/by/year
Deposited by: Memoria Investigacion
Deposited on: 14 Jul 2015 09:34
Last Modified: 21 May 2019 10:33
  • Logo InvestigaM (UPM)
  • Logo GEOUP4
  • Logo Open Access
  • Open Access
  • Logo Sherpa/Romeo
    Check whether the anglo-saxon journal in which you have published an article allows you to also publish it under open access.
  • Logo Dulcinea
    Check whether the spanish journal in which you have published an article allows you to also publish it under open access.
  • Logo de Recolecta
  • Logo del Observatorio I+D+i UPM
  • Logo de OpenCourseWare UPM