Populations genomics analysis of legume host preference for specific rhizobial genotypes in the Rhizobium leguminosarum bv. viciae symbioses

Jorrin, Beatriz and Imperial Ródenas, Juan (2015). Populations genomics analysis of legume host preference for specific rhizobial genotypes in the Rhizobium leguminosarum bv. viciae symbioses. "Molecular Plant-Microbe Interactions", v. 28 (n. 3); pp. 310-318. ISSN 0894-0282. https://doi.org/10.1094/MPMI-09-14-0296-FI.

Description

Title: Populations genomics analysis of legume host preference for specific rhizobial genotypes in the Rhizobium leguminosarum bv. viciae symbioses
Author/s:
  • Jorrin, Beatriz
  • Imperial Ródenas, Juan
Item Type: Article
Título de Revista/Publicación: Molecular Plant-Microbe Interactions
Date: March 2015
ISSN: 0894-0282
Volume: 28
Subjects:
Faculty: Centro de Investigación en Biotecnología y Genómica de Plantas (CBGP) (UPM)
Department: Biotecnologia [hasta 2014]
Creative Commons Licenses: Recognition - No derivative works - Non commercial

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Abstract

Rhizobium leguminosarum bv. viciae establishes root nodule symbioses with several legume genera. Although most isolates are equally effective in establishing symbioses with all host genera, previous evidence suggests that hosts select specific rhizobial genotypes among those present in the soil. We have used population genomics to further investigate this observation. P. sativum, L. culinaris, V. sativa, and V. faba plants were used to trap rhizobia from a well-characterized soil, and pooled genomic DNAs from one-hundred isolates from each plant were sequenced. Sequence reads were aligned to the R. leguminosarum bv. viciae 3841 reference genome. High overall conservation of sequences was observed in all subpopulations, although several multigenic regions were absent from the soil population. A large fraction (16-22%) of sequence reads could not be recruited to the reference genome, suggesting that they represent sequences specific to that particular soil population. Although highly conserved, the 16S-23S rRNA gene region presented single nucleotide polymorphisms (SNPs) regarding the reference genome, but no striking differences could be found among plant-selected subpopulations. Plant-specific SNP patterns were, however, clearly observed within the nod gene cluster, supporting the existence of a plant preference for specific rhizobial genotypes. This was also shown after genome-wide analysis of SNP patterns.

Funding Projects

TypeCodeAcronymLeaderTitle
Government of SpainCSD2009-00006UnspecifiedUnspecifiedUnspecified

More information

Item ID: 37469
DC Identifier: http://oa.upm.es/37469/
OAI Identifier: oai:oa.upm.es:37469
DOI: 10.1094/MPMI-09-14-0296-FI
Official URL: http://apsjournals.apsnet.org/doi/abs/10.1094/MPMI-09-14-0296-FI
Deposited by: Memoria Investigacion
Deposited on: 13 May 2016 14:35
Last Modified: 13 May 2016 14:35
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