Effect of the Legume Plant Host on Rhizobial Soil Population Dynamics

Soenens, Amalia and Jorrin, Beatriz and Imperial Ródenas, Juan (2014). Effect of the Legume Plant Host on Rhizobial Soil Population Dynamics. In: "Workshop: Genomics of prokaryotes, Handling the data analysis bottleneck. Glimpses at Pan -Genomes", 05/11/2014-07/11/2014, Valencia, Spain. p. 1.

Description

Title: Effect of the Legume Plant Host on Rhizobial Soil Population Dynamics
Author/s:
  • Soenens, Amalia
  • Jorrin, Beatriz
  • Imperial Ródenas, Juan
Item Type: Presentation at Congress or Conference (Article)
Event Title: Workshop: Genomics of prokaryotes, Handling the data analysis bottleneck. Glimpses at Pan -Genomes
Event Dates: 05/11/2014-07/11/2014
Event Location: Valencia, Spain
Title of Book: Genomics of prokaryotes, handling the data analysis bottleneck. Glimpses at Pan - Genomes
Date: 2014
Subjects:
Faculty: Centro de Investigación en Biotecnología y Genómica de Plantas (CBGP) (UPM)
Department: Otro
Creative Commons Licenses: Recognition - No derivative works - Non commercial

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Abstract

Rhizobium leguminosarum bv viciae (Rlv) is a soil bacterium able to establish specific root-nodule symbioses with legumes of four different genera: Pisum, Vicia, Lens and Lathyrus. Rlv isolates from nodules of any of these legumes can nodulate any of them; however, it has been shown that plants select specific rhizobial genotypes from those present in the soil (1,2). We have previously shown this at the genomic level by following a population genomics approach. Pool genomic sequences from 100 isolates from each of four plant species: P. sativum, L. culinaris, V. faba and V. sativa, show different, specific profiles at the single nucleotide polymorphism (SNP) level for relevant genes. In this work, the extent of Rlv selection from a well-characterized soil population by different legume plant hosts: P. sativum, L. culinaris, V. faba and V. sativa, after a medium-term mesocosm study is described. Direct soil isolates from each of these mesocosm studies have been tested for specific rhizobial genes (glnII and fnrN) and symbiotic genes (nodC and nifH). Different populations were characterized further by Sanger sequencing of both the rpoB phylogenetic marker gene and the symbiotic genes nodC and nifH. The distribution and size of the rhizobial population for each legume host showed changes during the medium-term mesocosm study. Particularly, a non-symbiotic group of rhizobia was enriched by all four hosts, in contrast to the symbiotic rhizobia profile, which was specific for each legume plant host.

More information

Item ID: 39275
DC Identifier: http://oa.upm.es/39275/
OAI Identifier: oai:oa.upm.es:39275
Official URL: http://fisabio.san.gva.es/web/fisabio/evento/-/asset_publisher/u2EU/content/workshop-genomica
Deposited by: Memoria Investigacion
Deposited on: 13 May 2016 15:02
Last Modified: 13 May 2016 15:02
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