In vitro maturation of guinea pig oocytes supplemented with Epidermal Growth Factor and Insulin-Like Growth Factor I

Cañón Beltrán, K. and Cajas, Y.N. and Carrera, R. and Lorenzo, P.L. and García Rebollar, Pilar and García García, R. M. and Arias Álvarez, M. (2016). In vitro maturation of guinea pig oocytes supplemented with Epidermal Growth Factor and Insulin-Like Growth Factor I. In: "30th Annual Meeting of the Brazilian Embryo Technology Society", 09/09/2016-10/09/2016, Barcelona. p. 1.

Description

Title: In vitro maturation of guinea pig oocytes supplemented with Epidermal Growth Factor and Insulin-Like Growth Factor I
Author/s:
  • Cañón Beltrán, K.
  • Cajas, Y.N.
  • Carrera, R.
  • Lorenzo, P.L.
  • García Rebollar, Pilar
  • García García, R. M.
  • Arias Álvarez, M.
Item Type: Presentation at Congress or Conference (Article)
Event Title: 30th Annual Meeting of the Brazilian Embryo Technology Society
Event Dates: 09/09/2016-10/09/2016
Event Location: Barcelona
Title of Book: 30th Annual Meeting of the Brazilian Embryo Technology Society
Date: 2016
Subjects:
Faculty: E.T.S. de Ingeniería Agronómica, Alimentaria y de Biosistemas (UPM)
Department: Producción Agraria
Creative Commons Licenses: Recognition - No derivative works - Non commercial

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Abstract

Insights in oocyte maturation process in guinea pigs are essential for the development of in vitro culture systems in this species, since it represents an interesting animal model in reproduction field (Suzuki et al. Mol Reprod Dev 2003; 64, 219–25). The goal of this study was to elucidate the influence of both Epidermal Growth Factor (EGF) and Insulin-like Growth Factor I (IGF-I) on in vitro oocyte maturation (IVM) medium of guinea pig. We assessed meiotic and cytoplasmic oocyte maturation, in terms of cortical granules (CG) and mitochondrial distribution, apoptotic rate and steroidogenic response of cumulus-oocyte-complexes (COCs) after IVM. A pool of 500 COCs from adult guinea pigs were cultured in groups of 40 COCs in four replicates in TCM-199 with 2 mM/mL glutamine, 0.1 mg/mL sodium pyruvate and 0.003% BSA for 17h (38ºC, 5%CO2) (Sigma Chemical Company).

More information

Item ID: 46618
DC Identifier: http://oa.upm.es/46618/
OAI Identifier: oai:oa.upm.es:46618
Official URL: http://www.sbte.org.br/_sbtEN.php?op=paginas&tipo=secao&secao=20&pagina=110
Deposited by: Memoria Investigacion
Deposited on: 20 Jun 2017 15:07
Last Modified: 20 Jun 2017 15:07
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