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Lara, Pilar and Ortego, Felix and González Hidalgo, Elena and Castañera, Pedro and Carbonero Zalduegui, Pilar and Diaz Rodriguez, Isabel (2000). Adaptation of Spodoptera exigua (Lepidoptera: Noctuidae) to barley trypsin inhibitor BTI-CMe expressed in transgenic tobacco. "Transgenic Research", v. 9 (n. 3); pp. 169-178. ISSN 0962-8819. https://doi.org/10.1023/A:100890563.
Title: | Adaptation of Spodoptera exigua (Lepidoptera: Noctuidae) to barley trypsin inhibitor BTI-CMe expressed in transgenic tobacco |
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Author/s: |
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Item Type: | Article |
Título de Revista/Publicación: | Transgenic Research |
Date: | June 2000 |
ISSN: | 0962-8819 |
Volume: | 9 |
Subjects: | |
Faculty: | E.T.S. de Ingeniería Agronómica, Alimentaria y de Biosistemas (UPM) |
Department: | Biotecnología - Biología Vegetal |
Creative Commons Licenses: | Recognition - No derivative works - Non commercial |
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Nicotiana tabacum plants were transformed with the cDNA of barley trypsin inhibitor BTI-CMe under the control of the 35S CaMV promoter. Although the transgene was expressed and the protein was active in the homozygous lines selected, growth of Spodoptera exigua (Lepidoptera: Noctuidae) larvae reared on transgenic plants was not affected. The protease activity in larval midgut extracts after 2 days feeding on transformed tobacco leaves from the highest expressing plant showed a reduction of 25% in the trypsin-like activity compared to that from insects fed on non-transformed controls. The susceptibility of digestive serine-proteases to inhibition by BTI-CMe was confirmed by activity staining gels. This decrease was compensated with a significant induction of leucine aminopeptidase-like and carboxipeptidase A-like activities, whilechymotrypsin-, elastase-, and carboxipeptidase B-like proteases were not affected.
Type | Code | Acronym | Leader | Title |
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Government of Spain | BIO96-2303 | Unspecified | Pilar Carbonero Zalduegui | Identificación y mutagenización de genes de cereales para su utilización transgénica frente a plagas de insectos |
Government of Spain | AGF98-0805C02-01 | Unspecified | Unspecified | Unspecified |
Madrid Regional Government | 07B/0006/98 | Unspecified | Isabel Díaz | Identificación y mutagenización de genes inhibidores de proteasas para la obtención de plantas transgénicas resistentes a insectos |
Item ID: | 53942 |
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DC Identifier: | http://oa.upm.es/53942/ |
OAI Identifier: | oai:oa.upm.es:53942 |
DOI: | 10.1023/A:100890563 |
Official URL: | https://link.springer.com/article/10.1023/A:1008905631440 |
Deposited by: | Biblioteca ETSI Agrónomos |
Deposited on: | 12 Feb 2019 12:03 |
Last Modified: | 12 Feb 2019 12:03 |