Adaptation of Spodoptera exigua (Lepidoptera: Noctuidae) to barley trypsin inhibitor BTI-CMe expressed in transgenic tobacco

Lara, Pilar and Ortego, Felix and González Hidalgo, Elena and Castañera, Pedro and Carbonero Zalduegui, Pilar and Diaz Rodriguez, Isabel (2000). Adaptation of Spodoptera exigua (Lepidoptera: Noctuidae) to barley trypsin inhibitor BTI-CMe expressed in transgenic tobacco. "Transgenic Research", v. 9 (n. 3); pp. 169-178. ISSN 0962-8819. https://doi.org/10.1023/A:100890563.

Description

Title: Adaptation of Spodoptera exigua (Lepidoptera: Noctuidae) to barley trypsin inhibitor BTI-CMe expressed in transgenic tobacco
Author/s:
  • Lara, Pilar
  • Ortego, Felix
  • González Hidalgo, Elena
  • Castañera, Pedro
  • Carbonero Zalduegui, Pilar
  • Diaz Rodriguez, Isabel
Item Type: Article
Título de Revista/Publicación: Transgenic Research
Date: June 2000
ISSN: 0962-8819
Volume: 9
Subjects:
Faculty: E.T.S. de Ingeniería Agronómica, Alimentaria y de Biosistemas (UPM)
Department: Biotecnología - Biología Vegetal
Creative Commons Licenses: Recognition - No derivative works - Non commercial

Full text

[img]
Preview
PDF - Requires a PDF viewer, such as GSview, Xpdf or Adobe Acrobat Reader
Download (3MB) | Preview

Abstract

Nicotiana tabacum plants were transformed with the cDNA of barley trypsin inhibitor BTI-CMe under the control of the 35S CaMV promoter. Although the transgene was expressed and the protein was active in the homozygous lines selected, growth of Spodoptera exigua (Lepidoptera: Noctuidae) larvae reared on transgenic plants was not affected. The protease activity in larval midgut extracts after 2 days feeding on transformed tobacco leaves from the highest expressing plant showed a reduction of 25% in the trypsin-like activity compared to that from insects fed on non-transformed controls. The susceptibility of digestive serine-proteases to inhibition by BTI-CMe was confirmed by activity staining gels. This decrease was compensated with a significant induction of leucine aminopeptidase-like and carboxipeptidase A-like activities, whilechymotrypsin-, elastase-, and carboxipeptidase B-like proteases were not affected.

Funding Projects

TypeCodeAcronymLeaderTitle
Government of SpainBIO96-2303UnspecifiedPilar Carbonero ZaldueguiIdentificación y mutagenización de genes de cereales para su utilización transgénica frente a plagas de insectos
Government of SpainAGF98-0805C02-01UnspecifiedUnspecifiedUnspecified
Madrid Regional Government07B/0006/98UnspecifiedIsabel DíazIdentificación y mutagenización de genes inhibidores de proteasas para la obtención de plantas transgénicas resistentes a insectos

More information

Item ID: 53942
DC Identifier: http://oa.upm.es/53942/
OAI Identifier: oai:oa.upm.es:53942
DOI: 10.1023/A:100890563
Official URL: https://link.springer.com/article/10.1023/A:1008905631440
Deposited by: Biblioteca ETSI Agrónomos
Deposited on: 12 Feb 2019 12:03
Last Modified: 12 Feb 2019 12:03
  • Logo InvestigaM (UPM)
  • Logo GEOUP4
  • Logo Open Access
  • Open Access
  • Logo Sherpa/Romeo
    Check whether the anglo-saxon journal in which you have published an article allows you to also publish it under open access.
  • Logo Dulcinea
    Check whether the spanish journal in which you have published an article allows you to also publish it under open access.
  • Logo de Recolecta
  • Logo del Observatorio I+D+i UPM
  • Logo de OpenCourseWare UPM