Barley BLZ1: a bZIP transcriptional activator that interacts with endosperm-specific gene promoters

Vicente Carbajosa, Jesus and Oñate Sanchez, Luis and Lara, Pilar and Diaz Rodriguez, Isabel and Carbonero Zalduegui, Pilar (1998). Barley BLZ1: a bZIP transcriptional activator that interacts with endosperm-specific gene promoters. "Plant Journal", v. 13 (n. 5); pp. 629-640. ISSN 0960-7412. https://doi.org/10.1111/j.1365-313X.1998.00068.x.

Description

Title: Barley BLZ1: a bZIP transcriptional activator that interacts with endosperm-specific gene promoters
Author/s:
  • Vicente Carbajosa, Jesus
  • Oñate Sanchez, Luis
  • Lara, Pilar
  • Diaz Rodriguez, Isabel
  • Carbonero Zalduegui, Pilar
Item Type: Article
Título de Revista/Publicación: Plant Journal
Date: March 1998
ISSN: 0960-7412
Volume: 13
Subjects:
Faculty: E.T.S. de Ingeniería Agronómica, Alimentaria y de Biosistemas (UPM)
Department: Biotecnología - Biología Vegetal
Creative Commons Licenses: Recognition - No derivative works - Non commercial

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Abstract

A cDNA encoding a bZIP transcription factor was obtainedfrom barley endosperm and used to identify the corres-ponding gene from a genomic library. The gene, designatedBlz1, contained six exons and five introns, plus a 442 nt-long 59-untranslated leader sequence, and was located onchromosome 5H. TheBlz1mRNA was detected early inendosperm development and was also expressed in rootsand leaves. The BLZ1 protein was a potent transcriptionalactivator in a yeast system; 85% of its activity was associ-ated with the first 203 amino acid residues at the N-terminus, which included two acidic regions. Presumptiveinvolvement ofBlz1in the regulation of gene expressionin endosperm was ascertained by the DNA-binding proper-ties of BLZ1 in electrophoretic mobility shift assays (EMSA)and by transient expression in barley developing endo-sperms, using, as effectors,Blz1in both sense and anti-sense orientations. In the co-bombardment experiments,theβ-glucuronidase (GUS) reporter gene responded toBlz1if under the control of the endosperm-specificItr1promoter or under a synthetic promoter containing theendosperm box of geneHor2. Sucrose synthase promotersSs1andSs2and synthetic promoters containing mutatedsequences ofHor2were unaffected in trans byBlz1.

Funding Projects

TypeCodeAcronymLeaderTitle
Government of SpainBIO94-0404UnspecifiedUnspecifiedUnspecified

More information

Item ID: 53966
DC Identifier: http://oa.upm.es/53966/
OAI Identifier: oai:oa.upm.es:53966
DOI: 10.1111/j.1365-313X.1998.00068.x
Official URL: https://onlinelibrary.wiley.com/doi/epdf/10.1111/j.1365-313X.1998.00068.x
Deposited by: Biblioteca ETSI Agrónomos
Deposited on: 12 Feb 2019 16:28
Last Modified: 12 Feb 2019 16:28
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