A New Device Based on Interferometric Optical Detection Method for Label-Free Screening of C-Reactive Protein

Maigler López, Mª Victoria and Holgado Bolaños, Miguel and Laguna Heras, Maria Fe and Sanza Gutiérrez, Francisco Javier and Santamaría Fernández, Beatriz and Lavin Hueros, Alvaro and López Espinosa, Rocío (2018). A New Device Based on Interferometric Optical Detection Method for Label-Free Screening of C-Reactive Protein. "IEEE Transactions on Instrumentation and Measurement" ; pp. 1-7. ISSN 1557-9662. https://doi.org/10.1109/TIM.2018.2876073.

Description

Title: A New Device Based on Interferometric Optical Detection Method for Label-Free Screening of C-Reactive Protein
Author/s:
  • Maigler López, Mª Victoria
  • Holgado Bolaños, Miguel
  • Laguna Heras, Maria Fe
  • Sanza Gutiérrez, Francisco Javier
  • Santamaría Fernández, Beatriz
  • Lavin Hueros, Alvaro
  • López Espinosa, Rocío
Item Type: Article
Título de Revista/Publicación: IEEE Transactions on Instrumentation and Measurement
Date: 2018
Subjects:
Freetext Keywords: C-reactive protein (CRP); label-free optical biosensors; point-of-care (PoC) devices; screening of immunoassays
Faculty: E.T.S.I. Industriales (UPM)
Department: Otro
Creative Commons Licenses: Recognition - No derivative works - Non commercial

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Abstract

In previous work, the performance of a compact and cost-effective point-of-care (PoC) device based on the increase relative optical power (IROP) methodology is reported and it is determined the enhancement in comparison with standard high-resolution spectrometry in terms of limit of detection. This paper describes a new label-free IROP-based biomedical device capable of working with low concentration of reagents and low sample volume per measurement in order to be used for screening different steps necessary in immunoassay optimization. This new approach significantly improves the sensing performance in terms of read-out signal (AIROP) per nanometer of biofilm in comparison with our previous work. This improvement is achieved due to the implementation of a laser as light source of the optical read-out system and the redesign of Fabry-Perot transducers by optimizing their reflectivity response and reducing their sensing area. For demonstrating the screening capability of this new PoC device in several immunoassays steps and methodologies, a C-reactive protein detection assay was carried out as a potential application and assay model. It is remarkable that only 10 μL of sample was used per measurement. This labelfree IROP-based device complies an easy-to-use and cost-effective tool for immunoassays optimization in terms of performance, reagents cost, and measuring time.

Funding Projects

TypeCodeAcronymLeaderTitle
Horizon 2020H2020-NMBP-2017UnspecifiedSabrina GrassiniUnspecified

More information

Item ID: 54829
DC Identifier: http://oa.upm.es/54829/
OAI Identifier: oai:oa.upm.es:54829
DOI: 10.1109/TIM.2018.2876073
Deposited by: Memoria Investigacion
Deposited on: 09 May 2019 15:08
Last Modified: 09 May 2019 15:08
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