Inhibition of eukaryotic cell-free protein synthesis by thionins from wheat endosperm

García Olmedo, Francisco and Carbonero Zalduegui, Pilar and Hernandez Lucas, Carlos and Paz Ares, Javier and Ponz Ascaso, Fernando and Vicente, O. (1983). Inhibition of eukaryotic cell-free protein synthesis by thionins from wheat endosperm. "Biochimica et Biophysica Acta", v. 740 (n. 1); pp. 52-56. ISSN 0006-3002. https://doi.org/10.1016/0167-4781(83)90120-3.

Description

Title: Inhibition of eukaryotic cell-free protein synthesis by thionins from wheat endosperm
Author/s:
  • García Olmedo, Francisco
  • Carbonero Zalduegui, Pilar
  • Hernandez Lucas, Carlos
  • Paz Ares, Javier
  • Ponz Ascaso, Fernando
  • Vicente, O.
Item Type: Article
Título de Revista/Publicación: Biochimica et Biophysica Acta
Date: May 1983
ISSN: 0006-3002
Volume: 740
Subjects:
Freetext Keywords: Protein synthesis; Thionin inhibition; Plant toxin; (Wheat endosperm)
Faculty: E.T.S.I. Agrónomos (UPM) [antigua denominación]
Department: Biotecnologia [hasta 2014]
Creative Commons Licenses: Recognition - No derivative works - Non commercial

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Abstract

Thionins are polypeptide toxins of about 5000 molecular weight, present in the endosperms of many Gramineae, which modify membrane permeability and inhibit macromolecular synthesis in cultured mammalian cells. Evidence is presented that they inhibit in vitro protein synthesis at micromolar concentrations in cell-free systems derived from wheat germ or from rabbit reticulocytes. Inhibition seems to occur by direct binding of mRNA by the toxin, as judged by the ability of thionins to mediate retention of RNA in nitrocellulose filters and by the dependence of inhibitory concentrations on the amount of exogenous RNA added to the wheat-germ translation system. Commercial preparations of wheat-germ have been found to include some endosperm contamination (up to 15%), which may result in at least partially inhibitory concentrations of the toxin in the cell-free extracts.

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