Inhibition of eukaryotic cell-free protein synthesis by thionins from wheat endosperm

García Olmedo, Francisco; Carbonero Zalduegui, Pilar; Hernandez Lucas, Carlos; Paz Ares, Javier; Ponz Ascaso, Fernando y Vicente, O. (1983). Inhibition of eukaryotic cell-free protein synthesis by thionins from wheat endosperm. "Biochimica et Biophysica Acta", v. 740 (n. 1); pp. 52-56. ISSN 0006-3002. https://doi.org/10.1016/0167-4781(83)90120-3.

Descripción

Título: Inhibition of eukaryotic cell-free protein synthesis by thionins from wheat endosperm
Autor/es:
  • García Olmedo, Francisco
  • Carbonero Zalduegui, Pilar
  • Hernandez Lucas, Carlos
  • Paz Ares, Javier
  • Ponz Ascaso, Fernando
  • Vicente, O.
Tipo de Documento: Artículo
Título de Revista/Publicación: Biochimica et Biophysica Acta
Fecha: Mayo 1983
Volumen: 740
Materias:
Palabras Clave Informales: Protein synthesis; Thionin inhibition; Plant toxin; (Wheat endosperm)
Escuela: E.T.S.I. Agrónomos (UPM) [antigua denominación]
Departamento: Biotecnologia [hasta 2014]
Licencias Creative Commons: Reconocimiento - Sin obra derivada - No comercial

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Resumen

Thionins are polypeptide toxins of about 5000 molecular weight, present in the endosperms of many Gramineae, which modify membrane permeability and inhibit macromolecular synthesis in cultured mammalian cells. Evidence is presented that they inhibit in vitro protein synthesis at micromolar concentrations in cell-free systems derived from wheat germ or from rabbit reticulocytes. Inhibition seems to occur by direct binding of mRNA by the toxin, as judged by the ability of thionins to mediate retention of RNA in nitrocellulose filters and by the dependence of inhibitory concentrations on the amount of exogenous RNA added to the wheat-germ translation system. Commercial preparations of wheat-germ have been found to include some endosperm contamination (up to 15%), which may result in at least partially inhibitory concentrations of the toxin in the cell-free extracts.

Más información

ID de Registro: 5940
Identificador DC: http://oa.upm.es/5940/
Identificador OAI: oai:oa.upm.es:5940
Identificador DOI: 10.1016/0167-4781(83)90120-3
URL Oficial: http://www.sciencedirect.com/science?_ob=ArticleURL&_udi=B6T1V-47S68XR-9S&_user=885385&_coverDate=05/20/1983&_rdoc=1&_fmt=high&_orig=search&_origin=search&_sort=d&_docanchor=&view=c&_acct=C000047350&_version=1&_urlVersion=0&_userid=885385&md5=ca4824f0fc1f
Depositado por: Memoria Investigacion
Depositado el: 09 Feb 2011 08:50
Ultima Modificación: 20 Abr 2016 14:37
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