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Nigrosine staining of wheat endosperm proteolipid patterns on starch gels
Cita
Aragoncillo Ballesteros, Cipriano, Rodriguez Loperena, M.A., Carbonero Zalduegui, Pilar 
ORCID: https://orcid.org/0000-0002-4949-3577 and García Olmedo, Francisco
(1975).
Nigrosine staining of wheat endosperm proteolipid patterns on starch gels.
"Analytical biochemistry", v. 63
(n. 2);
pp. 603-606.
ISSN 0003-2697.
https://doi.org/10.1016/0003-2697(75)90387-5.
ORCID: https://orcid.org/0000-0002-4949-3577 and García Olmedo, Francisco
(1975).
Nigrosine staining of wheat endosperm proteolipid patterns on starch gels.
"Analytical biochemistry", v. 63
(n. 2);
pp. 603-606.
ISSN 0003-2697.
https://doi.org/10.1016/0003-2697(75)90387-5.
Descripción
| Título: | Nigrosine staining of wheat endosperm proteolipid patterns on starch gels |
|---|---|
| Autor/es: |
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| Tipo de Documento: | Artículo |
| Título de Revista/Publicación: | Analytical biochemistry |
| Fecha: | Febrero 1975 |
| ISSN: | 0003-2697 |
| Volumen: | 63 |
| Número: | 2 |
| Materias: | |
| ODS: | |
| Escuela: | E.T.S.I. Agrónomos (UPM) [antigua denominación] |
| Departamento: | Biotecnologia [hasta 2014] |
| Licencias Creative Commons: | Reconocimiento - Sin obra derivada - No comercial |
Resumen
We have previously characterized a group of proteolipids from wheat endosperm, designated CM proteins, which are soluble in chloroformmethanol (2:1, v/v) and have a molecular weight lower than 25,000 daltons (1-3). These have been also studied by Redman and Ewart (4). The CM proteins are suitably fractionated into several components by starch gel electrophoresis at pH 3.2 (1). A sensitive staining procedure was required in connection with genetic studies of these proteins because phenotypes had to be ascertained in small endosperm fractions dissected without impairing normal germination and plant development. We report here on Nigrosine staining conditions for CM proteins under which high sensitivity and selectivity are achieved
Más información
| ID de Registro: | 5962 |
|---|---|
| Identificador DC: | https://oa.upm.es/5962/ |
| Identificador OAI: | oai:oa.upm.es:5962 |
| URL Portal Científico: | https://portalcientifico.upm.es/es/ipublic/item/9754316 |
| Identificador DOI: | 10.1016/0003-2697(75)90387-5 |
| URL Oficial: | http://www.sciencedirect.com/science?_ob=ArticleUR... |
| Depositado por: | Memoria Investigacion |
| Depositado el: | 14 Feb 2011 08:49 |
| Ultima Modificación: | 12 Nov 2025 00:00 |
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