Nigrosine staining of wheat endosperm proteolipid patterns on starch gels

Aragoncillo Ballesteros, Cipriano and Rodriguez Loperena, M.A. and Carbonero Zalduegui, Pilar and García Olmedo, Francisco (1975). Nigrosine staining of wheat endosperm proteolipid patterns on starch gels. "Analytical biochemistry", v. 63 (n. 2); pp. 603-606. ISSN 0003-2697. https://doi.org/10.1016/0003-2697(75)90387-5.

Description

Title: Nigrosine staining of wheat endosperm proteolipid patterns on starch gels
Author/s:
  • Aragoncillo Ballesteros, Cipriano
  • Rodriguez Loperena, M.A.
  • Carbonero Zalduegui, Pilar
  • García Olmedo, Francisco
Item Type: Article
Título de Revista/Publicación: Analytical biochemistry
Date: February 1975
ISSN: 0003-2697
Volume: 63
Subjects:
Faculty: E.T.S.I. Agrónomos (UPM) [antigua denominación]
Department: Biotecnologia [hasta 2014]
Creative Commons Licenses: Recognition - No derivative works - Non commercial

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Abstract

We have previously characterized a group of proteolipids from wheat endosperm, designated CM proteins, which are soluble in chloroformmethanol (2:1, v/v) and have a molecular weight lower than 25,000 daltons (1-3). These have been also studied by Redman and Ewart (4). The CM proteins are suitably fractionated into several components by starch gel electrophoresis at pH 3.2 (1). A sensitive staining procedure was required in connection with genetic studies of these proteins because phenotypes had to be ascertained in small endosperm fractions dissected without impairing normal germination and plant development. We report here on Nigrosine staining conditions for CM proteins under which high sensitivity and selectivity are achieved

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