Nucleotide sequence and endosperm-specific expression of the structural gene for the thionin alpha-hordothionin in barley (Hordeum vulgare L.)

Rodriguez Palenzuela, Pablo and Pintor Toro, Jose Antonio and Carbonero Zalduegui, Pilar and García Olmedo, Francisco (1988). Nucleotide sequence and endosperm-specific expression of the structural gene for the thionin alpha-hordothionin in barley (Hordeum vulgare L.). "Gene", v. 70 (n. 2); pp. 271-281. ISSN 0378-1119. https://doi.org/10.1016/0378-1119(88)90199-0.

Description

Title: Nucleotide sequence and endosperm-specific expression of the structural gene for the thionin alpha-hordothionin in barley (Hordeum vulgare L.)
Author/s:
  • Rodriguez Palenzuela, Pablo
  • Pintor Toro, Jose Antonio
  • Carbonero Zalduegui, Pilar
  • García Olmedo, Francisco
Item Type: Article
Título de Revista/Publicación: Gene
Date: October 1988
ISSN: 0378-1119
Volume: 70
Subjects:
Freetext Keywords: combinant DNA; genomic DNA clones; plant toxin; tramcriptional start point; oligodeoxynucleotide primer; promoter; phage λ; genomic library
Faculty: E.T.S.I. Agrónomos (UPM) [antigua denominación]
Department: Biotecnologia [hasta 2014]
Creative Commons Licenses: Recognition - No derivative works - Non commercial

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Abstract

A barley genomic library, obtained by cloning in the vector λEMBL-4, was screened with a cDNA probe encoding the α-hordothionin toxin. A positive clone, designated λTH1, was selected for further characterization. The coding and flanking regions of the α-hordothionin gene (Hth-1) were sequenced. Hth-1 has two introns of 420 and 91 nucleotides (nt), respectively. The promoter region has the following main features: one TATA box; three CATC boxes; an enhancer-like sequence, starting at nt position −282 from the first ATG codon, which is homologous to sequences appearing at similar positions in other endosperm genes; two versions of an 18-nt sequence that is more highly repeated in structural domains of several prolamin genes; two extensive regions close to the first ATG codon that are homologous to a sequence located much further upstream in the B-hordein promoter. The transcription start point was determined at nt positions −46 to −47, both by the S1 nuclease-protection and by the primer-extension assays. A maximum of 2–4 copies of the Hth-1 gene per haploid genome was determined by Southern-blot hybridization. Expression of the Hth-1 gene was detected during the cell proliferation stage of endosperm development (maximum at 13–16 days after pollinization) and was not detected in either etiolated or green coleoptiles

More information

Item ID: 6060
DC Identifier: http://oa.upm.es/6060/
OAI Identifier: oai:oa.upm.es:6060
DOI: 10.1016/0378-1119(88)90199-0
Official URL: https://www.sciencedirect.com/science/article/pii/0378111988901990
Deposited by: Memoria Investigacion
Deposited on: 17 Feb 2011 11:04
Last Modified: 07 Nov 2018 07:56
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