Two-dimensional electrophoresis as a tool for structural and genetic studies of seed proteins from Poaceae and Fagaceae.

García Olmedo, Francisco; Salcedo Duran, Gabriel; Aragoncillo Ballesteros, Cipriano; Sánchez-Monge Laguna de Rins, Rosa; Collada Collada, Maria Carmen y Gómez, Luis (1988). Two-dimensional electrophoresis as a tool for structural and genetic studies of seed proteins from Poaceae and Fagaceae.. "Electrophoresis", v. 9 (n. 11); pp. 719-727. ISSN 0173-0835. https://doi.org/10.1002/elps.1150091107.

Descripción

Título: Two-dimensional electrophoresis as a tool for structural and genetic studies of seed proteins from Poaceae and Fagaceae.
Autor/es:
  • García Olmedo, Francisco
  • Salcedo Duran, Gabriel
  • Aragoncillo Ballesteros, Cipriano
  • Sánchez-Monge Laguna de Rins, Rosa
  • Collada Collada, Maria Carmen
  • Gómez, Luis
Tipo de Documento: Artículo
Título de Revista/Publicación: Electrophoresis
Fecha: 1988
Volumen: 9
Materias:
Escuela: E.T.S.I. Agrónomos (UPM) [antigua denominación]
Departamento: Biología Vegetal [hasta 2014]
Licencias Creative Commons: Reconocimiento - Sin obra derivada - No comercial

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Resumen

The application of two-dimensional electrophoretic procedures to structural and genetic studies of seed proteins from Poaceae (including the cultivated cereals) and Fagaceae is described. The following related problems have been considered: covalent and non-covalent association of protein subunits in multiple oligomeric structures; chromosomal locations of genes encoding seed proteins; quantitation of gene products in relation to gene expression and regulation; purification of protein components to study their homology relationships and in vitro activities; evolutionary and phylogenetic relationships; identification of genetic stocks. Isoelectric focusing, pore-gradient electrophoresis, electrophoresis at different pH's, are among the separation procedures used in the first dimension, whereas sodium dodecyl sulfate-polyacrylamide gel electrophoresis and starch-gel electrophoresis at acid pH have been the preferred second-dimensional methods. Dissociating conditions (sodium dodecyl sulfate, Nonidet P-40, or urea) and reducing conditions (2-mercaptoethanol) have been used when required

Más información

ID de Registro: 6062
Identificador DC: http://oa.upm.es/6062/
Identificador OAI: oai:oa.upm.es:6062
Identificador DOI: 10.1002/elps.1150091107
URL Oficial: http://onlinelibrary.wiley.com/doi/10.1002/elps.1150091107/abstract
Depositado por: Memoria Investigacion
Depositado el: 17 Feb 2011 11:12
Ultima Modificación: 20 Abr 2016 14:42
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