Two-dimensional electrophoresis as a tool for structural and genetic studies of seed proteins from Poaceae and Fagaceae.

García Olmedo, Francisco and Salcedo Duran, Gabriel and Aragoncillo Ballesteros, Cipriano and Sánchez-Monge Laguna de Rins, Rosa and Collada Collada, Maria Carmen and Gómez, Luis (1988). Two-dimensional electrophoresis as a tool for structural and genetic studies of seed proteins from Poaceae and Fagaceae.. "Electrophoresis", v. 9 (n. 11); pp. 719-727. ISSN 0173-0835. https://doi.org/10.1002/elps.1150091107.

Description

Title: Two-dimensional electrophoresis as a tool for structural and genetic studies of seed proteins from Poaceae and Fagaceae.
Author/s:
  • García Olmedo, Francisco
  • Salcedo Duran, Gabriel
  • Aragoncillo Ballesteros, Cipriano
  • Sánchez-Monge Laguna de Rins, Rosa
  • Collada Collada, Maria Carmen
  • Gómez, Luis
Item Type: Article
Título de Revista/Publicación: Electrophoresis
Date: 1988
ISSN: 0173-0835
Volume: 9
Subjects:
Faculty: E.T.S.I. Agrónomos (UPM) [antigua denominación]
Department: Biología Vegetal [hasta 2014]
Creative Commons Licenses: Recognition - No derivative works - Non commercial

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Abstract

The application of two-dimensional electrophoretic procedures to structural and genetic studies of seed proteins from Poaceae (including the cultivated cereals) and Fagaceae is described. The following related problems have been considered: covalent and non-covalent association of protein subunits in multiple oligomeric structures; chromosomal locations of genes encoding seed proteins; quantitation of gene products in relation to gene expression and regulation; purification of protein components to study their homology relationships and in vitro activities; evolutionary and phylogenetic relationships; identification of genetic stocks. Isoelectric focusing, pore-gradient electrophoresis, electrophoresis at different pH's, are among the separation procedures used in the first dimension, whereas sodium dodecyl sulfate-polyacrylamide gel electrophoresis and starch-gel electrophoresis at acid pH have been the preferred second-dimensional methods. Dissociating conditions (sodium dodecyl sulfate, Nonidet P-40, or urea) and reducing conditions (2-mercaptoethanol) have been used when required

More information

Item ID: 6062
DC Identifier: http://oa.upm.es/6062/
OAI Identifier: oai:oa.upm.es:6062
DOI: 10.1002/elps.1150091107
Official URL: http://onlinelibrary.wiley.com/doi/10.1002/elps.1150091107/abstract
Deposited by: Memoria Investigacion
Deposited on: 17 Feb 2011 11:12
Last Modified: 20 Apr 2016 14:42
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