Characterization of beta-Nerve Growth Factor-TrkA system in male reproductive tract of rabbit and the relationship between beta-NGF and testosterone levels with seminal quality during sexual maturation

Sánchez Rodríguez, Ana and Arias Alvárez, María and Timón, Patricia and Bautista, J. M. and Garcia Rebollar, Pilar and Lorenzo González, Pedro Luis and García García, Rosa María (2019). Characterization of beta-Nerve Growth Factor-TrkA system in male reproductive tract of rabbit and the relationship between beta-NGF and testosterone levels with seminal quality during sexual maturation. "Theriogenology", v. 126 ; pp. 206-213. ISSN 0093-691X. https://doi.org/10.1016/j.theriogenology.2018.12.013.

Description

Title: Characterization of beta-Nerve Growth Factor-TrkA system in male reproductive tract of rabbit and the relationship between beta-NGF and testosterone levels with seminal quality during sexual maturation
Author/s:
  • Sánchez Rodríguez, Ana
  • Arias Alvárez, María
  • Timón, Patricia
  • Bautista, J. M.
  • Garcia Rebollar, Pilar
  • Lorenzo González, Pedro Luis
  • García García, Rosa María
Item Type: Article
Título de Revista/Publicación: Theriogenology
Date: March 2019
ISSN: 0093-691X
Volume: 126
Subjects:
Freetext Keywords: b-NGF; TrkA receptor; Seminal plasma; Sex organs; Testosterone levels; Male rabbits
Faculty: E.T.S. de Ingeniería Agronómica, Alimentaria y de Biosistemas (UPM)
Department: Producción Agraria
Creative Commons Licenses: Recognition - No derivative works - Non commercial

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Abstract

b-Nerve Growth Factor (b-NGF) is a neurotrophin which acts through its receptors TrkA and p75, performing important actions in male reproductive physiology and its presence in seminal plasma (SP) has been related to male fertility. The aim of the present study was to evaluate the gene expression profile and the immunolocalization ofb-NGF and its high-affinity receptor TrkA in sex organs in rabbits during sexual maturation period.b-NGF concentration for both SP and blood plasma (BP) and BP testosterone levels were determined as well as the seminal parameters during such period. Ten New Zealand White x California young rabbits were trained to semen collection since 20 weeks of age and routinely done once a week with two ejaculations per session. At 22 and 37 weeks of age, semen collection was carried out three times a week and seminal parameters were evaluated. Four males were randomly assigned and slaughtered in each age (n¼8); sex organs (prostate, bulbourethral glands and epididymis) were dissected and collected to determineb-NGF and TrkA gene expression and immunolocalization. SP and BP were also taken at each semen collection session to evaluateb-NGF concentration, and testosterone levels were also assessed in BP. The highestb-NGF mRNA expression was observed in prostate compared to bulbourethral glands and epididymis. These two last tissues showed residualb-NGF mRNA expression and limited localization of the neurotrophin. The prostate epithelial cells and lumen were strongly stained with regard to the other sex organs indicating that immunolocalization ofb-NGF rely mainly in the prostate. TrkA gene expression was lower but constant and differentially immunolocalized in the sex organ tissues. Finally,b-NGF concentration in SP and BP remained unchanged in accordance to age, while some seminal characteristics such as sperm concentration, percentage of live sperm and mass and progressive motility were enhanced as endowed by BP testosterone variation.b-NGF and its cognate TrkA receptor are expressed and immunolocalized in the male reproductive tract in the two ages studied, independently of the circulating levels of testosterone andb-NGF.

Funding Projects

TypeCodeAcronymLeaderTitle
Government of SpainAGL2015-65572-CUnspecifiedUnspecifiedEfecto de la restricción alimentaria materna en la respuesta ovárica y embrionaria y del papel del Factor de Crecimiento Nervioso (NGF) tras su caracterización en el conejo

More information

Item ID: 63656
DC Identifier: http://oa.upm.es/63656/
OAI Identifier: oai:oa.upm.es:63656
DOI: 10.1016/j.theriogenology.2018.12.013
Official URL: https://www.sciencedirect.com/science/article/pii/S0093691X18304552?via%3Dihub
Deposited by: Memoria Investigacion
Deposited on: 03 Nov 2020 09:44
Last Modified: 03 Nov 2020 09:44
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