Towards a digital model of zebrafish embryogenesis. Integration of Cell Tracking and Gene Expression Quantification

Castro González, Carlos; Luengo Oroz, Miguel Ángel; Douloquin, Louise; Savy, Thierry; Melani, Camilo; Desnoulez, Sophie; Ledesma Carbayo, María Jesús; Bourgine, Paul; Peyrieras, Nadine y Santos Lleo, Andres de (2010). Towards a digital model of zebrafish embryogenesis. Integration of Cell Tracking and Gene Expression Quantification. En: "32nd Annual International Conference of the IEEE Engineering in Medicine and Biology Society", 31/08/2010 - 04/09/2010, Buenos Aires, Argentina. ISBN 978-1-4244-4123-5.

Descripción

Título: Towards a digital model of zebrafish embryogenesis. Integration of Cell Tracking and Gene Expression Quantification
Autor/es:
  • Castro González, Carlos
  • Luengo Oroz, Miguel Ángel
  • Douloquin, Louise
  • Savy, Thierry
  • Melani, Camilo
  • Desnoulez, Sophie
  • Ledesma Carbayo, María Jesús
  • Bourgine, Paul
  • Peyrieras, Nadine
  • Santos Lleo, Andres de
Tipo de Documento: Ponencia en Congreso o Jornada (Artículo)
Título del Evento: 32nd Annual International Conference of the IEEE Engineering in Medicine and Biology Society
Fechas del Evento: 31/08/2010 - 04/09/2010
Lugar del Evento: Buenos Aires, Argentina
Título del Libro: Proceedings of the 32nd Annual International Conference of the IEEE Engineering in Medicine and Biology Society
Fecha: 2010
ISBN: 978-1-4244-4123-5
Materias:
Escuela: E.T.S.I. Telecomunicación (UPM)
Departamento: Ingeniería Electrónica
Licencias Creative Commons: Reconocimiento - Sin obra derivada - No comercial

Texto completo

[img]
Vista Previa
PDF (Document Portable Format) - Se necesita un visor de ficheros PDF, como GSview, Xpdf o Adobe Acrobat Reader
Descargar (879kB) | Vista Previa

Resumen

We elaborate on a general framework composed of a set of computational tools to accurately quantificate cellular position and gene expression levels throughout early zebrafish embryogenesis captured over a time-lapse series of in vivo 3D images. Our modeling strategy involves nuclei detection, cell geometries extraction, automatic gene levels quantification and cell tracking to reconstruct cell trajectories and lineage tree which describe the animal development. Each cell in the embryo is then precisely described at each given time t by a vector composed of the cell 3D spatial coordinates (x; y; z) along with its gene expression level g. This comprehensive description of the embryo development is used to assess the general connection between genetic expression and cell movement. We also investigate genetic expression propagation between a cell and its progeny in the lineage tree. More to the point, this paper focuses on the evolution of the expression pattern of transcriptional factor goosecoid (gsc) through the gastrulation process between 6 and 9 hours post fertilization (hpf)

Más información

ID de Registro: 6941
Identificador DC: http://oa.upm.es/6941/
Identificador OAI: oai:oa.upm.es:6941
URL Oficial: http://embc2010.embs.org/
Depositado por: Memoria Investigacion
Depositado el: 10 May 2011 11:46
Ultima Modificación: 20 Abr 2016 16:03
  • Open Access
  • Open Access
  • Sherpa-Romeo
    Compruebe si la revista anglosajona en la que ha publicado un artículo permite también su publicación en abierto.
  • Dulcinea
    Compruebe si la revista española en la que ha publicado un artículo permite también su publicación en abierto.
  • Recolecta
  • e-ciencia
  • Observatorio I+D+i UPM
  • OpenCourseWare UPM