Expression of a barley cystatin gene in maize enhances resistance against phytophagous mites by altering their cysteine-proteases

Carrillo Gil, Laura and Martinez Muñoz, Manuel and Ramessar, Koreen and Cambra Marin, Ines and Castañera, Pedro and Ortego, Felix and Diaz Rodriguez, Isabel (2011). Expression of a barley cystatin gene in maize enhances resistance against phytophagous mites by altering their cysteine-proteases. "Plant Cell Reports", v. 30 (n. 1); pp. 101-112. ISSN 0721-7714. https://doi.org/10.1007/s00299-010-0948-z.

Description

Title: Expression of a barley cystatin gene in maize enhances resistance against phytophagous mites by altering their cysteine-proteases
Author/s:
  • Carrillo Gil, Laura
  • Martinez Muñoz, Manuel
  • Ramessar, Koreen
  • Cambra Marin, Ines
  • Castañera, Pedro
  • Ortego, Felix
  • Diaz Rodriguez, Isabel
Item Type: Article
Título de Revista/Publicación: Plant Cell Reports
Date: January 2011
ISSN: 0721-7714
Volume: 30
Subjects:
Freetext Keywords: Cysteine protease – Phytocystatin – Spider mite – Transgenic maize – Tetranychus urticae – Brevipalpus chilensis
Faculty: E.T.S.I. Agrónomos (UPM) [antigua denominación]
Department: Biotecnologia [hasta 2014]
Creative Commons Licenses: Recognition - No derivative works - Non commercial

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Abstract

Phytocystatins are inhibitors of cysteine-proteases from plants putatively involved in plant defence based on their capability of inhibit heterologous enzymes. We have previously characterised the whole cystatin gene family members from barley (HvCPI-1 to HvCPI-13). The aim of this study was to assess the effects of barley cystatins on two phytophagous spider mites, Tetranychus urticae and Brevipalpus chilensis. The determination of proteolytic activity profile in both mite species showed the presence of the cysteine-proteases, putative targets of cystatins, among other enzymatic activities. All barley cystatins, except HvCPI-1 and HvCPI-7, inhibited in vitro mite cathepsin L- and/or cathepsin B-like activities, HvCPI-6 being the strongest inhibitor for both mite species. Transgenic maize plants expressing HvCPI-6 protein were generated and the functional integrity of the cystatin transgene was confirmed by in vitro inhibitory effect observed against T. urticae and B. chilensis protein extracts. Feeding experiments impaired on transgenic lines performed with T. urticae impaired mite development and reproductive performance. Besides, a significant reduction of cathepsin L-like and/or cathepsin B-like activities was observed when the spider mite fed on maize plants expressing HvCPI-6 cystatin. These findings reveal the potential of barley cystatins as acaricide proteins to protect plants against two important mite pests.

More information

Item ID: 7299
DC Identifier: http://oa.upm.es/7299/
OAI Identifier: oai:oa.upm.es:7299
DOI: 10.1007/s00299-010-0948-z
Official URL: http://www.springerlink.com/content/n9jj13uq3hg43qh2/
Deposited by: Memoria Investigacion
Deposited on: 01 Jun 2011 09:24
Last Modified: 20 Apr 2016 16:28
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