Expression of a barley cystatin gene in maize enhances resistance against phytophagous mites by altering their cysteine-proteases

Carrillo Gil, Laura; Martinez Muñoz, Manuel; Ramessar, Koreen; Cambra Marin, Ines; Castañera, Pedro; Ortego, Felix y Diaz Rodriguez, Isabel (2011). Expression of a barley cystatin gene in maize enhances resistance against phytophagous mites by altering their cysteine-proteases. "Plant Cell Reports", v. 30 (n. 1); pp. 101-112. ISSN 0721-7714. https://doi.org/10.1007/s00299-010-0948-z.

Descripción

Título: Expression of a barley cystatin gene in maize enhances resistance against phytophagous mites by altering their cysteine-proteases
Autor/es:
  • Carrillo Gil, Laura
  • Martinez Muñoz, Manuel
  • Ramessar, Koreen
  • Cambra Marin, Ines
  • Castañera, Pedro
  • Ortego, Felix
  • Diaz Rodriguez, Isabel
Tipo de Documento: Artículo
Título de Revista/Publicación: Plant Cell Reports
Fecha: Enero 2011
Volumen: 30
Materias:
Palabras Clave Informales: Cysteine protease – Phytocystatin – Spider mite – Transgenic maize – Tetranychus urticae – Brevipalpus chilensis
Escuela: E.T.S.I. Agrónomos (UPM) [antigua denominación]
Departamento: Biotecnologia [hasta 2014]
Licencias Creative Commons: Reconocimiento - Sin obra derivada - No comercial

Texto completo

[img]
Vista Previa
PDF (Document Portable Format) - Se necesita un visor de ficheros PDF, como GSview, Xpdf o Adobe Acrobat Reader
Descargar (1MB) | Vista Previa

Resumen

Phytocystatins are inhibitors of cysteine-proteases from plants putatively involved in plant defence based on their capability of inhibit heterologous enzymes. We have previously characterised the whole cystatin gene family members from barley (HvCPI-1 to HvCPI-13). The aim of this study was to assess the effects of barley cystatins on two phytophagous spider mites, Tetranychus urticae and Brevipalpus chilensis. The determination of proteolytic activity profile in both mite species showed the presence of the cysteine-proteases, putative targets of cystatins, among other enzymatic activities. All barley cystatins, except HvCPI-1 and HvCPI-7, inhibited in vitro mite cathepsin L- and/or cathepsin B-like activities, HvCPI-6 being the strongest inhibitor for both mite species. Transgenic maize plants expressing HvCPI-6 protein were generated and the functional integrity of the cystatin transgene was confirmed by in vitro inhibitory effect observed against T. urticae and B. chilensis protein extracts. Feeding experiments impaired on transgenic lines performed with T. urticae impaired mite development and reproductive performance. Besides, a significant reduction of cathepsin L-like and/or cathepsin B-like activities was observed when the spider mite fed on maize plants expressing HvCPI-6 cystatin. These findings reveal the potential of barley cystatins as acaricide proteins to protect plants against two important mite pests.

Más información

ID de Registro: 7299
Identificador DC: http://oa.upm.es/7299/
Identificador OAI: oai:oa.upm.es:7299
Identificador DOI: 10.1007/s00299-010-0948-z
URL Oficial: http://www.springerlink.com/content/n9jj13uq3hg43qh2/
Depositado por: Memoria Investigacion
Depositado el: 01 Jun 2011 09:24
Ultima Modificación: 20 Abr 2016 16:28
  • Open Access
  • Open Access
  • Sherpa-Romeo
    Compruebe si la revista anglosajona en la que ha publicado un artículo permite también su publicación en abierto.
  • Dulcinea
    Compruebe si la revista española en la que ha publicado un artículo permite también su publicación en abierto.
  • Recolecta
  • e-ciencia
  • Observatorio I+D+i UPM
  • OpenCourseWare UPM