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Effects of polylinker uATGs on the function of grass HKT1 transporters expressed in yeast cells
Citation
Bañuelos Bernabe, Maria Antonia and Haro Hidalgo, Rosario and Fraile-Escanciano, Ana and Rodriguez Navarro, Alonso (2008). Effects of polylinker uATGs on the function of grass HKT1 transporters expressed in yeast cells. "Plant and Cell Physiology", v. 49 (n. 7); pp. 1128-1132. ISSN 0032-0781. https://doi.org/10.1093/pcp/pcn088.
Description
| Title: | Effects of polylinker uATGs on the function of grass HKT1 transporters expressed in yeast cells |
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| Author/s: |
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| Item Type: | Article |
| Título de Revista/Publicación: | Plant and Cell Physiology |
| Date: | July 2008 |
| ISSN: | 0032-0781 |
| Volume: | 49 |
| Subjects: | |
| Freetext Keywords: | Plant, Potassium, Sodium, Transport, uORFs, Yeast. |
| Faculty: | E.T.S.I. Agrónomos (UPM) [antigua denominación] |
| Department: | Biotecnologia [hasta 2014] |
| Creative Commons Licenses: | Recognition - No derivative works - Non commercial |
Abstract
HvHKT1 mediates K+ or Na+ uniport in yeast cells if the expression promoter is joined directly to the HvHKT1 cDNA, and Na+-K+ symport if a 59-nt polylinker is inserted. Our results show that three ATG triplets in the polylinker decreased the synthesis of the transporter and that the lower amount of transporter caused the functional change. With the rice HKT1 cDNA, the 59-nt polylinker changed the mode of Na+ uptake from K+-insensitive to K+-inhibitable. These two modes of Na+ uptake also occurred in rice plants
More information
| Item ID: | 2353 |
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| DC Identifier: | https://oa.upm.es/2353/ |
| OAI Identifier: | oai:oa.upm.es:2353 |
| DOI: | 10.1093/pcp/pcn088 |
| Official URL: | http://pcp.oxfordjournals.org/content/vol49/issue7... |
| Deposited by: | Memoria Investigacion |
| Deposited on: | 19 Feb 2010 14:03 |
| Last Modified: | 20 Apr 2016 12:05 |
