Effects of polylinker uATGs on the function of grass HKT1 transporters expressed in yeast cells

Bañuelos Bernabe, Maria Antonia ORCID: https://orcid.org/0000-0002-0924-754X, Haro Hidalgo, Rosario ORCID: https://orcid.org/0000-0003-3528-9169, Fraile-Escanciano, Ana and Rodriguez Navarro, Alonso (2008). Effects of polylinker uATGs on the function of grass HKT1 transporters expressed in yeast cells. "Plant and Cell Physiology", v. 49 (n. 7); pp. 1128-1132. ISSN 0032-0781. https://doi.org/10.1093/pcp/pcn088.

Descripción

Título: Effects of polylinker uATGs on the function of grass HKT1 transporters expressed in yeast cells
Autor/es:
Tipo de Documento: Artículo
Título de Revista/Publicación: Plant and Cell Physiology
Fecha: Julio 2008
ISSN: 0032-0781
Volumen: 49
Número: 7
Materias:
ODS:
Palabras Clave Informales: Plant, Potassium, Sodium, Transport, uORFs, Yeast.
Escuela: E.T.S.I. Agrónomos (UPM) [antigua denominación]
Departamento: Biotecnologia [hasta 2014]
Licencias Creative Commons: Reconocimiento - Sin obra derivada - No comercial

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Resumen

HvHKT1 mediates K+ or Na+ uniport in yeast cells if the expression promoter is joined directly to the HvHKT1 cDNA, and Na+-K+ symport if a 59-nt polylinker is inserted. Our results show that three ATG triplets in the polylinker decreased the synthesis of the transporter and that the lower amount of transporter caused the functional change. With the rice HKT1 cDNA, the 59-nt polylinker changed the mode of Na+ uptake from K+-insensitive to K+-inhibitable. These two modes of Na+ uptake also occurred in rice plants

Más información

ID de Registro: 2353
Identificador DC: https://oa.upm.es/2353/
Identificador OAI: oai:oa.upm.es:2353
URL Portal Científico: https://portalcientifico.upm.es/es/ipublic/item/5483113
Identificador DOI: 10.1093/pcp/pcn088
URL Oficial: http://pcp.oxfordjournals.org/content/vol49/issue7...
Depositado por: Memoria Investigacion
Depositado el: 19 Feb 2010 14:03
Ultima Modificación: 12 Nov 2025 00:00