Repression of drought-induced cysteine-protease genes alters barley leaf structure and responses to abiotic and biotic stresses

Gómez Sánchez, Andrea and González Melendi, Pablo and Santamaría Fernández, María Estrella and Arbona Mengual, Vicente and López Gonzálvez, Ángeles and García, Antonia and Hensel, Goetz and Kumlehn, Jochen and Martinez Muñoz, Manuel and Diaz Rodriguez, Isabel (2019). Repression of drought-induced cysteine-protease genes alters barley leaf structure and responses to abiotic and biotic stresses. "Journal of Experimental Botany", v. 70 (n. 7); pp. 2143-2155. ISSN 0022-0957. https://doi.org/10.1093/jxb/ery410.

Description

Title: Repression of drought-induced cysteine-protease genes alters barley leaf structure and responses to abiotic and biotic stresses
Author/s:
  • Gómez Sánchez, Andrea
  • González Melendi, Pablo
  • Santamaría Fernández, María Estrella
  • Arbona Mengual, Vicente
  • López Gonzálvez, Ángeles
  • García, Antonia
  • Hensel, Goetz
  • Kumlehn, Jochen
  • Martinez Muñoz, Manuel
  • Diaz Rodriguez, Isabel
Item Type: Article
Título de Revista/Publicación: Journal of Experimental Botany
Date: March 2019
ISSN: 0022-0957
Volume: 70
Subjects:
Freetext Keywords: Abiotic/biotic stress; cysteine-protease; drought; leaf cuticles; stomata; water deprivation
Faculty: Centro de Investigación en Biotecnología y Genómica de Plantas (CBGP) (UPM)
Department: Otro
Creative Commons Licenses: Recognition - No derivative works - Non commercial

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Abstract

To survive under water deficiency, plants alter gene expression patterns, make structural and physiological adjustments, and optimize the use of water. Rapid degradation and turnover of proteins is required for effective nutrient recycling. Here, we examined the transcriptional responses of the C1A cysteine protease family to drought in barley and found that four genes were up-regulated in stressed plants. Knock-down lines for the protease-encoding genes HvPap-1 and HvPap-19 showed unexpected changes in leaf cuticle thickness and stomatal pore area. The efficiency of photosystem II and the total amount of proteins were almost unaltered in stressed transgenic plants while both parameters decreased in stressed wild-type plants. Although the patterns of proteolytic activities in the knock-down lines did not change, the amino acid accumulation increased in response to drought, concomitant with a higher ABA content. Whilst jasmonic acid (JA) and JA-Ile concentrations increased in stressed leaves of the wild-type and the HvPap-1 knock-down lines, their levels were lower in the HvPap-19 knock-down lines, suggesting the involvement of a specific hormone interaction in the process. Our data indicate that the changes in leaf cuticle thickness and stomatal pore area had advantageous effects on leaf defense against fungal infection and mite feeding mediated by Magnaporthe oryzae and Tetranychus urticae, respectively.

Funding Projects

TypeCodeAcronymLeaderTitle
Government of SpainBIO2104-53508-RUnspecifiedUnspecifiedSenescencia foliar en cebada: asociación entre la degradación-movilización de proteínas inducida por estrés abiótico/biótico y la calidad del grano

More information

Item ID: 63297
DC Identifier: https://oa.upm.es/63297/
OAI Identifier: oai:oa.upm.es:63297
DOI: 10.1093/jxb/ery410
Official URL: https://doi.org/10.1093/jxb/ery410
Deposited by: Memoria Investigacion
Deposited on: 10 Dec 2020 11:15
Last Modified: 03 Mar 2021 08:11
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