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Cerna Vargas, Jean Paul ORCID: https://orcid.org/0000-0001-5489-0385, Santamaría Hernándo, Saray
ORCID: https://orcid.org/0000-0001-6763-3839, Matilla Vázquez, Miguel Ángel, Rodríguez Herva, Jose Juan
ORCID: https://orcid.org/0000-0001-9962-2261, Daddaoua, Abdelali, Rodriguez Palenzuela, Pablo
ORCID: https://orcid.org/0000-0002-4963-9177, Krell, Tino and Lopez Solanilla, Emilia
(2019).
Chemoperception of Specific Amino Acids Controls Phytopathogenicity in Pseudomonas syringae pv. tomato.
"mBio", v. 10
(n. 5);
pp..
ISSN 2150-7511.
https://doi.org/10.1128/mBio.01868-19.
Title: | Chemoperception of Specific Amino Acids Controls Phytopathogenicity in Pseudomonas syringae pv. tomato |
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Author/s: |
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Item Type: | Article |
Título de Revista/Publicación: | mBio |
Date: | October 2019 |
ISSN: | 2150-7511 |
Volume: | 10 |
Subjects: | |
Freetext Keywords: | chemoreceptors; Pseudomonas syringae; virulence |
Faculty: | Centro de Investigación en Biotecnología y Genómica de Plantas (CBGP) (UPM) |
Department: | Otro |
Creative Commons Licenses: | Recognition - No derivative works - Non commercial |
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Chemotaxis has been associated with the pathogenicity of bacteria in plants and was found to facilitate bacterial entry through stomata and wounds. However, knowledge regarding the plant signals involved in this process is scarce. We have addressed this issue using Pseudomonas syringae pv. tomato, which is a foliar pathogen that causes bacterial speck in tomato. We show that the chemoreceptor P. syringae pv. tomato PscA (PsPto-PscA) recognizes specifically and with high affinity l-Asp, l-Glu, and d-Asp. The mutation of the chemoreceptor gene largely reduced chemotaxis to these ligands but also altered cyclic di-GMP (c-di-GMP) levels, biofilm formation, and motility, pointing to cross talk between different chemosensory pathways. Furthermore, the PsPto-PscA mutant strain showed reduced virulence in tomato. Asp and Glu are the most abundant amino acids in plants and in particular in tomato apoplasts, and we hypothesize that this receptor may have evolved to specifically recognize these compounds to facilitate bacterial entry into the plant. Infection assays with the wild-type strain showed that the presence of saturating concentrations of d-Asp also reduced bacterial virulence.
Item ID: | 63736 |
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DC Identifier: | https://oa.upm.es/63736/ |
OAI Identifier: | oai:oa.upm.es:63736 |
DOI: | 10.1128/mBio.01868-19 |
Official URL: | https://mbio.asm.org/content/mbio/10/5/e01868-19.f... |
Deposited by: | Memoria Investigacion |
Deposited on: | 17 Dec 2020 11:00 |
Last Modified: | 17 Dec 2020 11:57 |